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Immortalized Human Microglia-SV40 (HMC-3)
Cat.No.: CSC-I2226Z
Species: homo sapiens
Morphology: Polygonal
Culture Properties: Adherent
- Specification
- Q & A
- Customer Review
Cat.No.
CSC-I2226Z
Description
The HMC3 cell line was established through SV40-dependent immortalization of a human fetal brain-derived primary microglia culture. HMC3 is a microglial cell that was isolated from the brain of a patient. This cell line was deposited by KH Krause (University of Geneva, Switzerland).
Species
homo sapiens
Recommended Medium
SuperCult® Immortalized Human Microglia Medium (Cat No.: CM-I2226Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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Is it CD68, TMEM119, P2Y12 and IBA1 positive?
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).
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