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Immortalized Human Bone Marrow Mesenchymal Stromal Cells-hTERT (iMSC3)
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Cat.No.
CSC-I9245L
Description
Human mesenchymal stromal cells have extensive potential in research: uses for these including tissue repair and tissue engineering, gene therapy, the study of mesenchymal tumor oncogenes, and the analysis of cell fate determination and differentiation. The iMSC#3 cells express markers found in both primary human mesenchymal stromal cells and the multipotent form: CD90, CD44, NT5E, THY1, ENG, alanyl aminopeptidase (ANPEP), integrin beta I (ITGB1), integrin alpha 5 (ITGA5), major histocompatibililty complex, class I, A and B (HLA-A and B), and activated leukocyte cell adhesion molecule (ALCAM). These cells have also shown the ability to differentiate into adipocytes and osteoblasts. Furthermore, the cells have been verified for normal copy number, and lacks chromosomal aberrations and tumorigenicity.
Species
Homo sapiens
Source
Bone marrow
Culture Properties
Adherent
Morphology
Spindle-shaped
Immortalization Method
Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene (pBabe-puro-hTERT)
Markers
CD44, NT5E, THY1, ENG, CD90
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
1) Human telomerase reverse transcriptase expression was verified with quantitative PCR;
2) Surface markers were determined with flow cytometry;
3) Gene expression profile was analysed using the Illumina WG-6 v2 Expression BeadChip and Illumina HumanHT12 v4;
4) Adiopocyte differentiation was detected with Oil Red O staining, while osteoblast differentiation was detected by staining cells with Alizarin Red and ALPL and quantification;
5) DNA methylation profiling was performed using the Illumina HumanMethylation450 BeadChip. Copy number verification was performed with high-resolution mapping using Affymetrix Genome-Wide Human SNP Array 6.0;
6) Karyotype analysis was performed by arresting cells during metaphase and subjecting chromosomes to G-banding and subsequent imaging;
7) Tumorgenicity was verified in vivo with subcutaneous injection into immunodeficient athymic mice;
8) miRNA expression profile was determined using high-throughput sequencing.
2) Surface markers were determined with flow cytometry;
3) Gene expression profile was analysed using the Illumina WG-6 v2 Expression BeadChip and Illumina HumanHT12 v4;
4) Adiopocyte differentiation was detected with Oil Red O staining, while osteoblast differentiation was detected by staining cells with Alizarin Red and ALPL and quantification;
5) DNA methylation profiling was performed using the Illumina HumanMethylation450 BeadChip. Copy number verification was performed with high-resolution mapping using Affymetrix Genome-Wide Human SNP Array 6.0;
6) Karyotype analysis was performed by arresting cells during metaphase and subjecting chromosomes to G-banding and subsequent imaging;
7) Tumorgenicity was verified in vivo with subcutaneous injection into immunodeficient athymic mice;
8) miRNA expression profile was determined using high-throughput sequencing.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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