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Immortalized Human Bone Marrow Mesenchymal Cells-hTERT
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Cat.No.
CSC-I9085L
Description
The Bone Marrow Mesenchymal Cells are important for the growth and differentiation of primitive haematopoietic cells, as well as for maintenance of leukaemic cells. The immortalized cell was created by infecting primary mesenchymal cells with retrovirus containing hTERT and GFP genes separated by IRES under the control of MSCV LTR. The Immortalized Bone Marrow Mesenchymal cells can support growth of leukaemic cell lines 380, REH, OP-1 and 697, in addition to support the expansion of CD34+ cells. This cell line is useful not only in supporting the growth of haematopoietic cells, but can also be used in studies concerning bone marrow microenvironment and environmental cues that regulate haematopoiesis.
Species
Homo sapiens
Source
Bone Marrow
Culture Properties
Adherent
Morphology
spindle-shaped
Immortalization Method
Serial passaging and transduction with recombinant VSV-G retrovirus carrying hTERT and GFP genes
Markers
CD44 and CD73. These cells also express markers of osteoblasts (alkaline phosphatise) and chondrocytes (incorporation of toluidine blue dye)
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
Immunofluorescence and RT-PCR were used to confirm the presence hTERT expression. 2. PCR based TRAP was used to assay telomerase activity.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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