M-07e

Specification
Technical Resources
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Cat.No.

CSC-C0249

Description

Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells

Organism

Human

Source

acute megakaryoblastic leukemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS + human GM-CSF (10ng/ml)

Culture Properties

suspension

Morphology

single round cells in suspension, a few cells are lightly adherent

Karyotype

Human near-diploid karyotype - 46(45-46)<2n>XX, t(11;21)(p11;p13), add(13)(p13), add(22)(p13)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Product Sheets

Datasheet

No 'Material Safety Data Sheet' available yet

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For research use only. Not for any other purpose.

Q & A
Customer Review

How do I refresh cell media?

To media change, warm up fresh culture media at 37°C in a water bath or incubator for at least 30 mins. Carefully pour the media from the flask into a waste pot containing some disinfectant. Immediately replace the media with 100 ml of fresh pre-warmed culture media and return to the CO2 37°C incubator.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Wide-range of applications

The tumor cell products are suitable for use in a variety of cancer models, including xenografts and patient-derived organoids.

14 Nov 2022

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