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M-07e

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Cat.No.
CSC-C0249
Description
Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells
Organism
Human
Karyotype
Human near-diploid karyotype - 46(45-46)<2n>XX, t(11;21)(p11;p13), add(13)(p13), add(22)(p13)
Source
acute megakaryoblastic leukemia
Culture Properties
suspension
Morphology
single round cells in suspension, a few cells are lightly adherent
Recommended Medium
80-90% SuperCult® IMDM or RPMI-1640 + 10%-20% h.i. FBS + IL-3 (10 ng/ml) or GM-CSF (10 ng/ml) or 10-20% vol conditioned medium of cell line 5637
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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