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L-428

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Cat.No.
CSC-C0322
Description
Established from the pleural effusion of a 37-year-old woman with Hodgkin lymphoma (stage IVB, nodular sclerosis, refractory, terminal) in 1978
Species
Human
Source
Hodgkin lymphoma
Recommended Medium
Culture Properties
suspension
Morphology
single cells in suspension or as clusters; polymorph cells, some cells have "spikes", 1-5% giant multinucleated cells
Karyotype
Human flat-moded hypertetraploid karyotype with 10% polyploidy - 94(75-99)<4n>XXXX, +2, -5, +6, -9, -9, +12, -13, +17, +4mar, +2r/dmin, del(1)(q13), der(2)t(2;4)(p14;q21)x2-3, add(5)(p11), add(6)(q24/25)x2, add(7)(q35)x2, del(7)(p14), add(9)(p22), del(11)
Quality Control
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD25 -, CD30 +, CD34 -
Viruses: ELISA: reverse
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

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Q:
What is DAPI staining used for?
A:

A simple-to-use fluorescent stain, 4',6-diamidino-2-phenylindole (DAPI), visualizes nuclear DNA in both living and fixed cells. DAPI staining was used to determine the number of nuclei and to assess gross cell morphology.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Adequate documentation

Creative Bioarray provides adequate documentation and reference material to support the use of this product in experiments.

14 Sep 2022


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