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Cynomolgus Monkey Dermal Fibroblasts

Cat.No.: CSC-C4723L

Species: Monkey

Source: Dermis; Skin

Cell Type: Fibroblast

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Cat.No.
CSC-C4723L
Description
Cynomolgus Monkey Primary Dermal Fibroblasts are isolated from normal Cynomolgus Monkey skin tissue.
Species
Monkey
Source
Dermis; Skin
Cell Type
Fibroblast
Disease
Normal
Quality Control
Cynomolgus Monkey Primary Dermal Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratins 18, and alpha smooth muscle actin. Cynomolgus Monkey Primary Dermal Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Cynomolgus monkey dermal fibroblasts are isolated from the dermal tissue of cynomolgus monkey. Fibroblasts are the primary cell type in the dermis and are involved in a range of physiological and pathological processes. For instance, fibroblasts are the main producers of the extracellular matrix (ECM) of the skin and can secrete various types of collagens (such as type I and III), elastin, fibronectin, and other ECM components to provide structural support for the skin. When tissues become inflamed or injured, fibroblast cells increase their activity by releasing numerous cytokines and chemokines which aid in tissue repair. Fibroblasts also possess immune regulatory functions, able to secrete various cytokines (such as IL-6, TGF-β, IL-10, etc.), regulate the activity of T cells, B cells, and macrophages, and influence immune responses. In addition, fibroblasts can also serve as antigen-presenting cells (APCs) and participate in immune responses in lymphoid tissues. In recent years, studies have shown that fibroblasts also exhibit some characteristics of stem cells, with the ability to self-renew and differentiate into multiple cell types under certain conditions. For example, fibroblasts can obtain characteristics similar to mesenchymal stem cells (MSCs) through epigenetic regulation and exhibit multipotent differentiation potential. Fibroblasts can also be reprogrammed into induced pluripotent stem cells (iPSCs), providing new tools for stem cell research and regenerative medicine.

Generation and Characterization of cynHLCs from Cynomolgus Monkey Dermal Fibroblasts

This study presents the development of a Pc in vitro infection model using stem cell-derived hepatocytes from Macaca fascicularis. Cynomolgus iPS (cynIPS) cells were generated from two Cynomolgus Monkey dermal fibroblasts by reprogramming, using Sendai viruses bearing the transcription factors Oct3/4, Sox2, Klf4, and c-Myc. Pluripotent colonies appeared two to three weeks after infection and were kept on irradiated mouse embryonic fibroblasts. After several passages, the iPS cells were transferred on Matrigel, but spontaneous differentiation occurred on this feeder-less system (Fig. 1). Further investigations showed that a higher concentration of bFGF (basic fibroblast growth factor) was essential to maintain pluripotency (Fig. 1). Following the clonal expansion phase, clones exhibiting typical human embryonic stem cell-like morphology (flat and tightly packed colonies of round cells with large nuclei) were selected and characterized by immunofluorescence (Fig. 1). Based on this result the best clone (#13) was selected and checked for karyotype (Fig. 1), and differentiation potential into the three germ layers (Fig. 1).

Generation and characterization of Cynomolgus nHLCs. Fig. 1. Generation and characterization of cynHLCs (Pellisson M, Zeeman A M, et al., 2021).

CERV1 Infectivity in Human and Primate Cell Lines

Endogenous retroviruses (ERVs) are ancient viral sequences in the mammalian genome, providing insights into virus–host co-evolution. Chimpanzee endogenous retrovirus 1 (CERV1), an extinct gamma retrovirus, has circulated in primates for about 10 million years. AbuEed et al. aim to understand how CERV1 was transmitted and endogenized in primates.

In the present study, CERV1 was found to be a viral interference group of PERV-A and PERV-A/C. As CERV1, PERV-A, and PERV-A/C utilize the same receptor, human and primate cell lines were assessed for susceptibility by Env-pseudotyped viruses (Fig. 2). CERV1 was found to infect humans (293T, HeLa, HT1080, and Hs68 cells), chimpanzees (Chimp-0363M cells), and African green monkeys (Cos7 and Vero cells), although not NCMDF (normal cynomolgus monkey dermal fibroblasts). Contrastingly, PERV-A was found to infect HEK293T and HeLa cells but not other lines. Moreover, with the exception of Vero cells, PERV-A/C was established to show infectivity in the human and primate cell lines assessed in this study. These findings indicate that despite using the same viral receptor, there are differences in susceptibility with respect to viral cell entry.

Comparison of the human and primate cell lines' infectivity of CERV1, PERV-A, and PERV-A/C.Fig. 2. Comparison of the infectivity of CERV1, PERV-A, and PERV-A/C in human and primate cell lines (AbuEed L, Miyake A, et al., 2025).

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