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Immortalized Human Corneal Epithelial Cells

Cat.No.: CSC-I1916Z

Species: homo sapiens

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I1916Z
Description
Primary human Corneal epithelial cells were isolated and transfected with full-length human telomerase reverse transcriptase (hTERT) expressing lentiviral particles. This immortalized human Corneal epithelial cells can that allows the establishment of a standardized, reproducible cornea barrier model.
Species
homo sapiens
Recommended Medium
SuperCult® Immortalized Human Corneal Epithelial Cell Medium (Cat No.: CM-I1916Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Human telomerase reverse transcriptase (hTERT)
Application
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Quality Control
Real Time PCR was used to quantify hTERT gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Cultured human corneal epithelial cell lines could provide an efficient model for the study of cellular signaling and molecular pathways regulating normal corneal epithelial cell homeostasis. Previously, cultured human cells lines have been developed for this purpose by transformation with viral oncoproteins including adenovirus E1A, the SV40 large T antigen, and HPV16-E6/E7. The effectiveness of these virally transformed cell lines as potential research models has been hampered by both genetic instability and a lack of normal growth and differentiation properties, preventing their effective use for studying normal epithelial cell biology.

Human cell lines immortalized with hTERT exhibit genetic stability, normal contact inhibition, and maintain the capacity to differentiate. hTERT-immortalized human corneal epithelial cells (HCEs) exhibit a high degree of morphological similarity to primary cultures, expresses a panel of markers associated with corneal epithelial cell function (e.g., ABCG2, cytokeratin 3 and PAX 6 as well as integrins α9 and β1) and respond to epidermal growth factor in proliferation, migration and scratch wound assays. hTERT-immortalized HCEs represents a useful tool for studying many aspects of corneal epithelial cell biology, including regeneration and the response to growth factors, drugs or toxicity, with the added benefit of being able to grow on uncoated substrates in the absence of media supplements typically required for similar cell lines.

L-carnitine Partially Restores Adherens Junction Integrity and Promotes Wound Healing in Human Corneal Epithelial Cells

Disruption of tear film homeostasis and increased osmolarity are key features of dry eye disease (DED), leading to inflammation, epithelial barrier dysfunction, and ocular surface damage. Adherens junctions, primarily composed of cadherins and catenins, are essential for maintaining epithelial integrity and modulating signaling pathways that regulate cell proliferation and migration. This study investigates the effects of hyperosmolarity on adherens junction proteins and wound healing, as well as the therapeutic potential of L-carnitine (LCAR) in mitigating these effects.

To induce hyperosmolar stress, telomerase-immortalized human corneal epithelial cells (hTCEpi) were treated with 70, 90, and 120 mM NaCl, resulting in final osmolarities of approximately 450, 490, and 550 mOsM, respectively. LCAR supplementation (200 mM) was evaluated as a potential osmoprotective therapy.

Hyperosmolarity caused a dose-dependent reduction in trans-epithelial resistance (TER), with a 30–69 % decline across treatment groups, along with significantly impaired cell migration. Adherens junction proteins (E-cadherin, β-catenin, and p120-catenin) were downregulated, while α-catenin was upregulated. Notably, L-carnitine treatment alleviated these effects, significantly restoring TER and adherens junction protein levels to near-normal. These findings demonstrate that hyperosmolarity impairs corneal epithelial barrier function and delays wound healing by altering adherens junction complex. The results highlight the potential of L-carnitine as a therapeutic agent to restore epithelial barrier integrity and mitigate hyperosmolarity-induced damage in DED.

Telomerase-immortalized human corneal epithelial cells (hTCEpi) treated with 70, 90, and 120 mM NaCl corresponding to final osmolarities of ∼450, 490, and 550 mOsM, respectively, for 24 h exhibited a dose-dependent reduction (30–69 %) in trans-epithelial electrical resistance (TER).

Fig. 1. Hyperosmolarity induces a dose-dependent decline in human corneal epithelial barrier function (Kontoh-Twumasi, Richard, et al., 2025).

L-carnitine (LCAR) supplementation significantly improved E-cadherin (A,B) and β-catenin (D,E) fluorescence intensity and RNA expression (C, F).

Fig. 2. L-carnitine supplementation partially restores hyperosmolarity-induced loss of adherens junction protein integrity (Kontoh-Twumasi, Richard, et al., 2025).

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For research use only. Not for any other purpose.