Established from the solid omental metastasis of a mucinous papillary adenocarcinoma of the ovary of a 36-year-old woman in 1979; initially diploid, later tetraploid; growth in nude mice
Human flat-moded hypotetraploid karyotype with 17% polyploidy - 78-91<4n>XXXX, -4, -10, -13, -13, -19, +mar - i(9q), 16qh+x2 - extensive subclonal rearrangements, e.g. der(5)t(5;7)(q31.2;q11)
adherent, epithelial-like cells growing as monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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