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Immortalized Rat Embryonic Striatal (M26-1F) Cells-tsSV40T
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Cat.No.
CSC-I9301L
Description
Immortalized Rat Embryonic Striatal (M26-1F) Cells - tsSV40T has GABAgeric properties with multiple nucleoli, presence of GAD and MAP-2 antigen, absence of vimentin, and no contact inhibition features.
It is a tool which can be used to study GABA uptake and release, neural development and differentiation, and is a good model for Huntington’s disease. The cells grown in permissive temperature (33°C), whereas in non-permissive temperature (39.5°C), the morphology of the cells will become multipolar. SV40 large T antigen is stably expressed at both temperatures compared to the Immortalized Rat Embryonic Striatal (M213-2O) Cells - tsSV40T.
It is a tool which can be used to study GABA uptake and release, neural development and differentiation, and is a good model for Huntington’s disease. The cells grown in permissive temperature (33°C), whereas in non-permissive temperature (39.5°C), the morphology of the cells will become multipolar. SV40 large T antigen is stably expressed at both temperatures compared to the Immortalized Rat Embryonic Striatal (M213-2O) Cells - tsSV40T.
Source
Straitum
Culture Properties
Adherent
Morphology
Fibroblast-like
Immortalization Method
Immortalized via infection with defective recombinant retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT004 HT® Lenti-SV40 (tsA58 temperature sensitive mutant) Lentivirus Immortalization Kit
CIK-HT004 HT® Lenti-SV40 (tsA58 temperature sensitive mutant) Lentivirus Immortalization Kit
Quality Control
1) Immunostaining for SV40 T large antigen and markers
2) Test growth in permissive and non-permissive temperatures
2) Test growth in permissive and non-permissive temperatures
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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