Immortalized Mouse Lung Epithelial Cells (MLE-12)
Cat.No.: CSC-I2116Z
Species: mouse
Morphology: Polygonal
Culture Properties: Adherent
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Cat.No.
CSC-I2116Z
Description
This line was established in 1992 by Kathryn A. Wikenheiser from pulmonary tumors in a mouse transgenic for the SV40 large T antigen under the control of the promoter region of the human surfactant protein C gene.The cells express the mRNA for large T antigen. Lung surfactant proteins B and C were detected. The cells secrete phospholipids in response to phorbol esters and ATP but not in response to forskolin.
Species
                                mouse
                            Recommended Medium
                                SuperCult® Immortalized Mouse Lung Epithelial Cell Medium (Cat No.: CM-I2116Z)
                            Freezing Medium
                                Complete medium supplemented with 10% (v/v) DMSO
                            Culture Properties
                                Adherent
                            Morphology
                                Polygonal
                            Immortalization Method
                                SV40 large T antigen
                            Growth Properties
                                Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
                            Shipping
                                Dry Ice.
                            Quality Control
                                Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
                            free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Storage and Shipping
                                Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
                            Note: Never can cells be kept at -20°C.
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