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Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1)
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Cat.No.
CSC-I9253L
Description
The Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1) are non-transformed cells derived from leptin-receptor (LepR) deficient mice which display some phenotypic and functional properties similar to primary macrophages. The cells are characterized as early differentiated macrophages since the high expression of MAC-2, and low expression of F4/80, Ly6G and Ly6C were observed; but CD11b expression was absent. It is recommended for use in studies involving the role of the leptin receptor, susceptibility to infections, innate immune functions, and obesity.
Species
Mus musculus
Source
Bone Marrow
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Spontaneous immortalization
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) qRT-PCR confirmed LepR deficiency;
2) Flow cytometry was used to analyze cell surface markers.
2) Flow cytometry was used to analyze cell surface markers.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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