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Immortalized Mouse Dopaminergic Neuronal Cells (MN9D)
Cat.No.: CSC-I2295Z
Species: mouse
Morphology: Polygonal
Culture Properties: Adherent
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Cat.No.
CSC-I2295Z
Description
Immortalized Mouse Dopaminergic Neuronal Cell line (MN9D) was generated by fusion between rostral mesencephalic neurons from a 14-day-old C57BL/6J mouse embryo and N18TG2 neuroblastoma cells, a sympathetic nervous system cancer of A/Jax background. MN9D cells produce dopamine (DA) and express tyrosine hydroxylase (TH) as well as aromatic amino acid decarboxylase (AADC). MN9D cells readily aggregate with one another or other embryonic brain cells and can be transfected using calcium phosphate precipitation or Lipofectamine. Undifferentiated or differentiated MN9D cells are extensively used to model dopaminergic neurons and to test mechanisms and potential therapeutics relevant to the loss of DA neurons in Parkinson's disease. Differentiated with butyric acid or glial cell line-derived neurotrophic factor (GDNF) followed by butyric acid, MN9D cells only partially recapitulate the electrophysiological properties of midbrain DA neurons. Optimizing MN9D differentiation further using one or a combination of growth or other factors may yield an improved model system for Parkinson's disease studies in vitro.
Species
mouse
Recommended Medium
SuperCult® Immortalized Mouse Dopaminergic Neuronal Cell Medium (Cat No.: CM-I2295Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Application
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
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