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Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND)
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Cat.No.
CSC-I9179L
Description
Homeostasis of the central nervous system (CNS) is maintained by the blood brain barrier (BBB) and disruptions to the BBB are linked to many disorders of the CNS. The Immortalized Mouse Cerebral Capillary Endothelial Cell Line (cEND) provides a useful model for studies involving the differentiation and regulation of BBB as it shares principal features of the BBBin vivo, specifically in that 1) it retains the endothelial markers VE-Cadherin and PECAM-1; 2) it displays tight junction associated markers such as occludin, claudin-3, -5, -12, 3) it expresses the BBB marker protein Glut-1 and 4) it shows high electrical resistance, representing barrier properties. This cell line is also responsive to glucocorticoid, estrogen-treatment and pro-inflammatory mediator such as TNFα, making this cell line valuable in elucidating cellular responses of endothelial cells to different stimuli. Together with the Immortalized Mouse Cerebellar Capillary Endothelial Cell Line (cerebEND), the two cell lines represent importantin vitromodel system for these different brain regions.
Species
Mus musculus
Source
Cerebral cortex
Culture Properties
Adherent
Morphology
spindle shaped
Immortalization Method
Transformation with oncoprotein of murine Polyomavirus, Polyoma middle T antigen (PymT)
Markers
Claudin-5, Occuldin, Glut-1, VE-Cadherin
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) Endothelial marker VE-Cadherin and PECAM-1 assessed by immunostainning;
2) Tight junction protein Claudin-5 assessed by immunostainning and claudin-1, -3 and -12 detected in mRNA level;
3) BBB marker protein Glut-1 and tight junction-associated protein occludin measured by western blot analysis.
The cell line's response to pro-inflammatory stimuli was measured by 1) TER, 2) western blot and 3) gene expression analysis using RT-PCR.
2) Tight junction protein Claudin-5 assessed by immunostainning and claudin-1, -3 and -12 detected in mRNA level;
3) BBB marker protein Glut-1 and tight junction-associated protein occludin measured by western blot analysis.
The cell line's response to pro-inflammatory stimuli was measured by 1) TER, 2) western blot and 3) gene expression analysis using RT-PCR.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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