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FKH-1

  • Specification
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Cat.No.
CSC-C0613
Description
Established in 1993 from the peripheral blood of a 61-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) with trilineage myelodysplasia at refractory leukemic transformation into acute myeloid leukemia (AML M4); carries t(6;9)(p23;q34) leading to DEK-NUP214 (DEK-CAN) fusion gene; cells do not respond to G-CSF, as originally described
Species
Human
Source
acute myelocytic leukemia
Recommended Medium
Culture Properties
suspension
Morphology
mostly single, round cells in suspension
Karyotype
Human hypodiploid karyotype with 4% polyploidy; 45(43-46)<2n>XY, -7, t(6;9)(p23;q34); carries t(6;9) effecting rearrangement of DEK with NUP214 (CAN); closely resembles published karyotype
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD11b +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
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Q:
What stage is best for karyotyping?
A:

For karyotyping the chromosomes must be isolated from cells in the metaphase, which is the stage of the cell cycle in which the chromosomes assume their characteristic condensed, discrete shape.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Helpful

We have been able to use this product to study the effects of various cancer mutations on cell behavior.

26 Feb 2022


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