FKH-1

Cat.No.: CSC-C0613

Species: Human

Source: acute myelocytic leukemia

Morphology: mostly single, round cells in suspension

Culture Properties: suspension

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0613

Description

Established in 1993 from the peripheral blood of a 61-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) with trilineage myelodysplasia at refractory leukemic transformation into acute myeloid leukemia (AML M4); carries t(6;9)(p23;q34) leading to DEK-NUP214 (DEK-CAN) fusion gene; cells do not respond to G-CSF, as originally described

Species

Human

Source

acute myelocytic leukemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

mostly single, round cells in suspension

Karyotype

Human hypodiploid karyotype with 4% polyploidy; 45(43-46)<2n>XY, -7, t(6;9)(p23;q34); carries t(6;9) effecting rearrangement of DEK with NUP214 (CAN); closely resembles published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD11b +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What stage is best for karyotyping?

For karyotyping the chromosomes must be isolated from cells in the metaphase, which is the stage of the cell cycle in which the chromosomes assume their characteristic condensed, discrete shape.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

Helpful

We have been able to use this product to study the effects of various cancer mutations on cell behavior.

26 Feb 2022

Ease of use
After sales services
Value for money

Write your own review

You May Also Need

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

MeMo 05

Description: Species: human male 66 years old; Tissue: lymph node, metastasis; Tumor: melanoma

Cat#: CSC-6296W INQUIRY

View Product

Daudi

Description: Species: human - male, 16 years old, BlackReceptor: complement; Fc of Ig GTumorigenecity: yes, , in nude mice; form colonies in agaroseIsoenzyme: G6PD, BKaryology: normal male; diploid; ...

Cat#: CSC-C9369L INQUIRY

View Product

REH

Description: Species: human (Homo sapiens)Cell type: B cell precursor leukemiaOrigin: established from the peripheral blood of a 15-year-old North African girl with acute lymphoblastic leukemia (ALL at first ...

Cat#: CSC-C0205 INQUIRY

View Product

ROV-S

Description: Species: human, Caucasian; Tissue: bone marrow; Transformed by: Epstein-Barr; Derived from: children with maturation defects of haematopoiesis

Cat#: CSC-6310W INQUIRY

View Product

SCC-3

Description: Human monocytic cell line expressing Interleukin-2 receptor.

Cat#: CSC-C6668J INQUIRY

View Product

UT-7

Description: established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7); cells are constitutively cytokine-dependent and responsive to various cytokines

Cat#: CSC-C0294 INQUIRY

View Product

For research use only. Not for any other purpose.