GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.
Hams F10 + 10% Heat-inactivated FCS + 2mM Glutamine + 2ng/ml basic fibroblast growth factor + 80µg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10µg/cm2 from a 0.01% solution in acetic acid. After re
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.
Liquid Nitrogen (-180 °C).
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