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KYSE-180

Cat.No.: CSC-C0427

Species: Human

Source: esophageal squamous cell carcinoma

Morphology: epitheloid cells growing as adherent monolayer

Culture Properties: monolayer

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Cat.No.
CSC-C0427
Description
Established from the well differentiated esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 53-year-old Japanese man prior to treatment (tumor was invading contiguous structures)
Species
Human
Source
esophageal squamous cell carcinoma
Recommended Medium
Culture Properties
monolayer
Morphology
epitheloid cells growing as adherent monolayer
Karyotype
Human hyperdiploid karyotype with 11% polyploidy - 55(50-57)<2n>XX, +1, +1, +3, +7, +7, +8, -14, +16, -17, -17, -18, -18, +5-8mar, del(X)(q23), add(1)(q32), der(1;6)(q10;p10), der(1;13)(q10;q10), add(3)(p26), add(5)(q25), add(6)(p11), del(6)(q23), add(7)(
Quality Control
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cy
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Derived from a primary esophageal squamous cell carcinoma (ESCC) of a Japanese patient in the 1990s, the KYSE-180 cell line is a well-characterized, widely used in vitro model for ESCC research. It exhibits typical epithelial morphology, carries common ESCC genetic alterations (including mutations in TP53 and CDKN2A), expresses squamous differentiation markers such as cytokeratins, and demonstrates moderate invasive and migratory capabilities.

Current research applications heavily leverage KYSE-180 for drug discovery and therapeutic testing, especially for screening novel chemotherapeutics, targeted therapies (e.g., against EGFR, PI3K/mTOR pathways), and immunotherapies (e.g., checkpoint inhibitors such as anti-PD-1/PD-L1, often used in combination studies). It plays an important role in studying the molecular pathways driving ESCC progression, metastasis, and treatment resistance (e.g., EMT, apoptosis evasion), and serves as a backbone for genetic manipulation studies (knockdown/overexpression) to validate gene function. Its key strengths include: strong representation of ESCC biology (mutation profile, marker expression), robustness in standard cell culture conditions, consistent growth properties facilitating high-throughput assays, and extensive use in xenograft models for in vivo validation. This combination of relevance, reliability, and operability makes KYSE-180 an indispensable tool for advancing our understanding and treatment of this aggressive malignancy.

The Genomic Landscape of Esophageal Squamous Cell Carcinoma (ESCC) Cell Lines

This study aims to elucidate the malignancy, invasion capability, classical cancer-related signaling pathways, and immune status of ESCC cell lines, providing a detailed genomic landscape and highlighting the unique features of each cell line.

Whole exome and RNA sequencing were conducted on ESCC cell lines TE-1, ECA-109, KYSE-30, KYSE-150, KYSE-180, KYSE-450, and KYSE-510, with the normal epithelium cell line Het-1a as a comparison. Bioinformatics methods analyzed gene mutation types, mutation frequencies, RNA expression, and classical cancer-related signaling pathways. Specific analyses were also performed on tumor burden, genes related to differentiation, invasion, immunity, and gene enrichment in each cell line.

(A) Gene mutation of classical cancer-related signaling pathways in ESCC cell lines. (B) Mutation frequency of classical cancer-related signaling pathways in ESCC cell lines. (C) Copy number variations (CNV) and RNA expression of classical cancer-related signaling pathways in ESCC cell lines.Fig. 1. Gene mutation in classical cancer-related signaling pathways (Zhang, Chao, et al. 2025).

(A) The expression of oncogenic related genes in ESCC cell lines. (B) Expression of invasion related genes in ESCC cell lines. (C) NRF2 expression in ESCC cell lines. (D) Expression of differentiation related genes in ESCC cell lines. (E) Expression of immune related genes in ESCC cell lines.Fig. 2. The expression of oncogenic related genes (Zhang, Chao, et al. 2025).

KLF13 Promotes Esophageal Cancer Progression

Kruppel-Like Factor 13 (KLF13) has strong effects on cancer occurrence and progression. Nevertheless, the role of KLF13 in esophageal cancer (EC) remain elusive. In this study, Pengjie Yang and his team detected the expression of KLF13 in EC tissues and cells using immunohistochemistry, western blot, and real-time PCR, and found that KLF13 was upregulated in EC tissues and cells compared to normal controls. High expression of KLF13 indicated a poor prognosis for EC patients. Further, function studies in vitro and in vivo were performed to explore the role of KLF13 in EC cell progression. The results revealed that KLF13 knockdown suppressed EC cell proliferation, migration, epithelial-mesenchymal transition, increased cell apoptosis and cell cycle arrest in vivo and inhibited tumor growth in vitro.

KLF13 is upregulated in EC.Fig. 3. KLF13 is highly expressed in EC (Yang, Pengjie, et al. 2025).

KLF13 silencing suppressed EC cell proliferation and migration.Fig. 4. Silencing KLF13 suppressed EC cell proliferation and migration (Yang, Pengjie, et al. 2025).

What is the concentration of serum to be used?

Serum is used as an additive to the synthetic medium, usually at a concentration of 5-20%, most often at 10%.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Thankful

I am extremely thankful to the staff in the Creative Bioarray for their help.

22 June 2022


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