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HCT-15

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Cat.No.
CSC-C0410
Description
Established from a colorectal adenocarcinoma; HCT-15 is a sister cell line of the cell line DLD-1. The HCT-15 cells are CSAp negative (CSAp-). They are positive for keratin by immunoperoxidase staining.
Tissue of Origin
Colon
Organism
Homo sapiens (human)
Recommended Medium
Culture Properties
Adherent
Morphology
epithelial
STR DNA Profile
Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
Karyotype
Human pseudodiploid karyotype with 16% polyploidy - 46<2n>XYq+, t(8;17) (p23;q12/21), inv(11)(p14.2;q13) - stable karyotypic differences cf DLD-1 - matches published karyotype
Applications
The HCT-15 cell line can be used for tumourigenicity studies and also can be used as a suitable transfection host.

Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -
Storage and Shipping
liquid nitrogen vapor phase
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
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Europe
Tel: 44-208-144-6005
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