HCT-15

Biogenic PR-Agnps Inhibit Cell Proliferation in Colon Cancer HCT-15 Cells

Biosynthesized nanoparticles show promise in improving chemotherapy effectiveness, but there is limited research on biogenic silver nanoparticles from terrestrial ferns. Datkhile et al. investigated the effects of silver nanoparticles synthesized from Pteridium revolutum on inhibiting proliferation and inducing apoptosis in HCT-15 cancer cells.

In vitro studies evaluated the inhibitory effects of biogenic PR-AgNPs on HCT-15 colon cancer cells. PR-AgNPs exhibited dose-dependent cytotoxicity (2.5-15.0 µg/mL), with cell viability decreasing as concentration increased (Fig. 1A). MTT assay results showed significant suppression of HCT-15 cell proliferation after 24 h, with IC₅₀ and IC₉₀ values of 5.79±0.58 µg/mL and 13.82±1.38 µg/mL, respectively. Complete growth inhibition was achieved at 15 µg/mL. Morphological changes, including cell shrinkage, were observed in HCT-15 cells treated with 10-15 µg/mL PR-AgNPs (Fig. 1B), contrasting with healthy control cells. PR-AgNPs induced intracellular suicide in HCT-15 cells, leading to compromised cell membrane integrity, cytoplasmic condensation, and apoptotic cell death. Normal fibroblast cells from breast cancer tissue exhibited low toxicity when exposed to PR-AgNPs. Even at 15 µg/mL, PR-AgNPs inhibited less than 40.70±11.83% of fibroblast cell growth after 24 h.