Established from a colorectal adenocarcinoma; HCT-15 is a sister cell line of the cell line DLD-1. The HCT-15 cells are CSAp negative (CSAp-). They are positive for keratin by immunoperoxidase staining.
Homo sapiens, human
STR DNA Profile
Human pseudodiploid karyotype with 16% polyploidy - 46<2n>XYq+, t(8;17) (p23;q12/21), inv(11)(p14.2;q13) - stable karyotypic differences cf DLD-1 - matches published karyotype
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -
Storage and Shipping
liquid nitrogen vapor phase
The HCT-15 cell line can be used for tumourigenicity studies and also can be used as a suitable transfection host.
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