CEA(-), CSAp(-), colon antigen 3(+); This line is a subline of COLO 320DM. The cells are weakly positive for keratins and vimentinTumorigenecity:yes, in nude miceIsoenzyme:PGM1, 1; PGM3, 2; G6PD, B; PEP-D, 1; 6PGD, A;ES-D, 1Histopathology:adenocarcinoma; DukesSubculture:Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol. Subculture once per week.Split ratio:Media change:Reverse transcritase:negativeProductionserotonin; norepinephrine; epinephrine; adrenocorticotropic hormone(ACTH); parathyroid hormone
STR DNA Profile
Penta E: 11
Penta D: 9/12
FGA: 20 , 2010.
The cells grows loosely attached and in suspension
rounded and refractile
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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