Adult Stem Cell Generation
Mesenchymal stem cells (MSCs), together with hematopoietic stem cells (HSCs), and other functional stem cells are frequently used cell types for cell-based therapeutics, drug screening, and pathology study of disease on cell models. Moreover, it is important to establish correctly typed cell lines from human tissue donations. Creative Bioarray provides service for isolating, and identifying MSCs, HSCs, and other adult stem cells from various tissues obtained through human tissue donation.
Application:
Mesenchymal stem cells (MSCs) have drawn great interest in the field of regenerative medicine, for cell replacement, immunomodulatory, and gene therapies. It has been shown that these multipotent stromal cells can be isolated from tissues such as bone marrow, adipose tissue, trimester amniotic tissue, umbilical cord blood, and deciduous teeth and can be expanded in adherent culture. They have the capacity to differentiate into cells of the connective tissue lineages in vitro and contribute to tissue parenchyma in vivo.
Figure 1. Immunomodulatory functions of MSCs.
Other adult stem cells are found in small numbers in most adult tissues, such as bone marrow or fat. They can help the body to heal itself because they play critical role in regeneration and therapy. As they can divide easily, they can replace dead and injured cells. Thus, adult stem cells can help to understand how diseases occur, use in regenerative medicine, and test new drug for safety and effectiveness.
Figure 2: Functional properties of cardiac stem cells.
Creative Bioarray Offers Adult Stem Cell Generation Service for Your Scientific Research as Follows (including but not limited to):
1. MSC Isolation and Characterization:
- Primary tissue recovery
- Isolation of MSC from bone marrow, adipose, umbilical cord, and other sources
- MSC purification
- Characterization of MSC, must express CD105, CD73, and CD90 and must lack expression of CD45, CD34, CD14, and HLA-DR
2. Other Adult Stem Cells Generation and Characterization:
- Adult stem cell generation and purification
- Characterization of adult stem cells with their specific markers
- Various cell types are supported:
- Hematopoietic stem cells
- Neural stem cells
- Pancreatic stem cells
- Kidney stem cells
- Gastric stem cells
- Other types of stem cells
Note: All services can be provided separately or as a package.
Advantages of Creative Bioarray Service:
- Worldwide accepted and advanced isolation and purification approaches
- Stable and pure cell lines
- Reliable cell sources
- Flexible service pattern to satisfy your demand
- Guaranteed service quality
- Detailed report of experiment conditions
Example for MSC and Characterization Results:
Figure 3: Phenotypic and functional characterization of MSCs. (A) The morphology of umbilical cord-derived MSC and their differentiation into adipogenic, chondrogenic, and osteogenic lineages. (B) Immunophenotypic characterization of UC-MSC by Flow Cytometry.
Figure 4: Immunocytochemical analysis of neural stem cells. Scale bar is 50μm.
Creative Bioarray is an experienced and outstanding provider of adult stem cell generation service. We are dedicated to providing detailed data and comprehensive service for your scientific research, and we are pleased to use our extensive experience and advanced platform to offer the best service to satisfy each demand from our customers.
If you have any special need in adult stem cell generation service, do not hesitate to contact us for this special service. Please let us know what you need and we will accommodate you. We are looking forward to working with you in the future.
References
Leite, et al. "Multipotent stem cells of the heart—do they have therapeutic promise?." Frontiers in Physiology 6 (2015): 123.
Shi, et al. "Immunomodulatory properties and therapeutic application of mesenchymal stem cells." Clinical & Experimental Immunology 164.1 (2011): 1-8.
D'Aiuto, et al. "Large-scale generation of human iPSC-derived neural stem cells/early neural progenitor cells and their neuronal differentiation." Organogenesis 10.4 (2014): 365-377.
