Somatic Cell Reprogramming

iPSC became a promising breakthrough in regenerative medicine since 2006. Because of its high pluripotency, differentiation ability, non-ethical, and -immune problem, iPSC technology provides great opportunity for cell modeling, drug selecting, and cell replacing.

Application: Currently, iPSCs could also be of great use in studying molecular mechanism of many diseases and production of patient-specific cells which can be transplanted to the site of injury or the site of tissue degeneration due to various disease conditions. Moreover, iPSC technology has been employed in various diseases for disease modeling and gene therapy. Many toxic compounds which are encountered by humans and newly designed drugs may be evaluated for toxicity and effects by using iPSCs. Thus, the applications of iPSCs in regenerative medicine, disease modeling, and drug discovery are enormous and should be explored in a more comprehensive manner.

Creative Bioarray Offers Reprogramming Service for Your Scientific Research as Follows (including but not limited to):

1. Cell Reprogramming:

• Primary cell recovery and expansion
• Reprogramming and cell culture when uniform iPSC morphology appear
• Pick and expand iPSC colonies

2. iPSC Validation:

• Characterization of iPSC for pluripotency markers, including: Oct4, Sox2, SSEA4, Tra1-81, and Nanog
• Karyotyping
• iPSC safety testing
• In vivo teratoma generation
• DNA methylation analysis

Note: All services can be provided separately or as a package.

Advantages of Creative Bioarray Service:

• Worldwide accepted reprogramming approach
• Non-integration to iPSC
• Over 95% rate of reprogramming
• Feed-free reprogramming service
• Guaranteed service quality

Example for Reprogramming Cell and Characterization Results:

Figure 1: The morphology of cells derived from fibroblast about two weeks after reprogramming.

Figure 2: Characterization result of iPSC by immunostaining with Oct4, Sox2, SSEA4, and Tra1-81.

Creative Bioarray is an experienced and outstanding provider of somatic cell reprogramming service. We are dedicated to providing detailed data and comprehensive service for your scientific research, and we are pleased to use our extensive experience and advanced platform to offer the best service to satisfy each demand from our customers.

If you have any special need in cell reprogramming from primary cells, do not hesitate to contact us for this special service. Please let us know what you need and we will accommodate you. We are looking forward to working with you in the future.

References

1. Takahashi, et al. "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors." cell 126.4 (2006): 663-676.

2. Csöbönyeiová, et al. "Toxicity testing and drug screening using iPSC-derived hepatocytes, cardiomyocytes, and neural cells." Canadian journal of physiology and pharmacology 94.7 (2016): 687-694.