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M-07e

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  • Specification
  • Technical Resources
  • Recommended Products
  • Cat.No.
  • CSC-C0249
  • Description
  • Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells
  • Recommended Medium
  • 80-90% SuperCult® IMDM or RPMI-1640 + 10%-20% h.i. FBS + IL-3 (10 ng/ml) or GM-CSF (10 ng/ml) or 10-20% vol conditioned medium of cell line 5637
  • Organism
  • Human
  • Morphology
  • single round cells in suspension, a few cells are lightly adherent
  • Culture Properties
  • suspension
  • Karyotype
  • Human near-diploid karyotype - 46(45-46)<2n>XX, t(11;21)(p11;p13), add(13)(p13), add(22)(p13)
  • Source
  • acute megakaryoblastic leukemia
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -
    Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, SMRV -
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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      Tel: 1-631-626-9181
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com