Human Adipose Microvascular Endothelial Cells (HAMEC)

Specification
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Cat.No.

CSC-7846W

Description

Microvascular endothelial cells line blood vessels and contribute to many biological processes such as angiogenesis, coagulation, trafficking of lymphocytes, and the inflammatory response. Microvascular endothelial cells are diverse and have specific cellular characteristics and functions depending on the organ/tissue in which they are found. Adipose tissue is unique because it has the capacity to continually grow throughout adult life. It thus has a high occurrence of angiogenesis in order to provide the extensive vascularization require for adipose tissue. Studies have shown that angiogenesis precedes adipogenesis, implying that microvascular endothelial cells influence the proliferation of preadipocytes. Microvascular endothelial cell growth is at the same time stimulated by adipocyte secreted VEGG, suggesting a complex paracrine relationships between microvascular endothelial cells and preadipocytes during tissue development.HAMEC from Bioarray Research Laboratories are isolated from human adipose tissue. HAMEC are cryopreserved after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HAMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HAMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAMEC are guaranteed to further expand for 15 population doublings at the conditions provided by Bioarray Research Laboratories.

Species

Human

Source

Adipose

Cell Type

Endothelial

Disease

Normal

Storage and Shipping

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

What are the benefits of Human Adipose Microvascular Endothelial Cells (HAMEC)?

Studies have shown that angiogenesis precedes adipogenesis, implying that microvascular endothelial cells influence the proliferation of preadipocytes. At the same time, microvascular endothelial cell growth is stimulated by adipocyte secreted VEGG, suggesting a complex paracrine relationship between microvascular endothelial cells and preadipocytes during tissue development.

What passage number would they be?

You will receive Human Adipose Microvascular Endothelial Cells (HAMEC) at passages 2 or 3, depending on cell purity.

Is there a recommended medium?

It is recommended to use SuperCult® Custom-Made Endothelial Cell Medium (cat# CM-1085X) for the culturing of HAMEC.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent reproducibility

The cell product has excellent reproducibility.

18 May 2022

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