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CAL-51

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  • Cat.No.
  • CSC-C0382
  • Description
  • Established from the pleural effusion metastasis of a 45-year-old woman with progressive breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype
  • Recommended Medium
  • 80% DMEM (4.5 g/L glucose) + 20% h.i. FBS
  • Organism
  • Human
  • Morphology
  • epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris
  • Culture Properties
  • monolayer
  • Karyotype
  • Human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vime
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast carcinoma

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com