Our Promise to You

Guaranteed product quality, expert customer support

Our Promise to You
Browse Cells
Need help to Filer Cell Lines  by Type, Tissue and  Species?

Human Dermal Fibroblasts-adult (HDF-a)

  • Specification
  • Recommended Products
Cat.No.
CSC-7798W
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. In addition, dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.
HDF-a are isolated from adult human skin. HDF-a are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HDF-a are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HDF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HDF-a are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Species
Human
Source
Dermis
Morphology
Fibroblasts
Applications
Human Primary Dermal Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Cell Type
Fibroblast
Disease
Normal
Quality Control
Human Primary Dermal Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Dermal Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
We ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
  • Customer Review

Ask a Question

Write your own review

Customer Support & Price Inquiry

Verification Code