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CAL-120

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  • Cat.No.
  • CSC-C0488
  • Description
  • Established from the pleural effusion of a 43-year-old woman with metastatic breast adenocarcinoma in 1991
  • Recommended Medium
  • 90% DMEM + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypertetraploid karyotype with 20% polyploidy 98(88-98)<4n>XXX/XXXX, -1, -2, -2, -4, +5, +5, +6, -9, -9, +10, +11, -12, -12, -13, -13, -14, -15, -16, -16, -20, -21, -22, +16-18mar, + 1-2 dmin, add(X)(p2?2), add(1)(q22), del(3)(q22), add(3)(p1?4), de
  • Quality Control
  • Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimen
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast adenocarcinoma

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com