HCEC-B4G12

Specification
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Cat.No.

CSC-C3457

Description

HCEC-B4G12 is a clonal subpopulation started in 2005 from the parental cell line HCEC-12 established from normal cells of the posterior epithelium of the cornea of a 91-year-old Caucasian woman transformed with the early region of the SV40 genome including genes encoding large T-antigen and small t-antigen; the subclone is described to represent differentiated cells of the so-called corneal endothelium

Species

Human

Source

corneal endothelium

Recommended Medium

Human-Endothelial-SFM + 10 ng/ml FGF-2 (observe the shelf-life of the medium; do not apply h.i. FBS in order to keep differentiation status)

Culture Properties

monolayer

Morphology

cuboid epitheloid cells growing strongly adherent in monolayers

Reference

Publication

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 (+), cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Viruses: P

Storage and Shipping

Frozen with 90% medium (including FGF-2), 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Product Sheets

Datasheet

No 'Material Safety Data Sheet' available yet

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Dong C, Li Z, Wang X, et al. SRT1720 attenuates UVA-induced corneal endothelial damage via inhibition of oxidative stress and cellular apoptosis[J]. Experimental Eye Research, 2023, 231: 109464.
Park J H, Lee K, Park C Y. Effect of magnetic microparticles on cultivated human corneal endothelial cells[J]. Translational Vision Science & Technology, 2023, 12(2): 14-14.
Song E S, Park J H, Ha S S, et al. Novel Corneal Endothelial Cell Carrier Couples a Biodegradable Polymer and a Mesenchymal Stem Cell-Derived Extracellular Matrix[J]. ACS Applied Materials & Interfaces, 2022, 14(10): 12116-12129.
Qiu J, Shan K, Xiang J, et al. Comprehensive Analysis of circRNA-Associated-ceRNA Networks in Human Corneal Endothelial Dysfunction[J]. Cornea, 2022, 41(12): 1545-1552.
Yuan S, Yuan X, Li L. Long non-coding RNA HOXA11-AS protects the barrier function of corneal endothelial cells by sponging microRNA-155 to alleviate corneal endothelial injury[J]. American Journal of Translational Research, 2022, 14(12): 8489.
Zhang X, Qiu J, Huang F, et al. Type 2 Diabetes Mellitus Makes Corneal Endothelial Cells Vulnerable to Ultraviolet A-Induced Oxidative Damage Via Decreased DJ-1/Nrf2/NQO1 Pathway[J]. Investigative Ophthalmology & Visual Science, 2022, 63(12): 25-25.
Wang Q, Dou S, Zhang B, et al. Heterogeneity of human corneal endothelium implicates lncRNA NEAT1 in Fuchs endothelial corneal dystrophy[J]. Molecular Therapy-Nucleic Acids, 2022, 27: 880-893.
Chen C, Zhang B, Xue J, et al. Pathogenic role of endoplasmic reticulum stress in diabetic corneal endothelial dysfunction[J]. Investigative Ophthalmology & Visual Science, 2022, 63(3): 4-4.
Li R, Qu Y, Li X, et al. Molecular Hydrogen Attenuated N-methyl-N-Nitrosourea Induced Corneal Endothelial Injury by Upregulating Anti-Apoptotic Pathway[J]. Investigative Ophthalmology & Visual Science, 2021, 62(9): 2-2.
Guo S P, Chang H C, Lu L S, et al. Activation of kelch-like ECH-associated protein 1/nuclear factor erythroid 2-related factor 2/antioxidant response element pathway by curcumin enhances the anti-oxidative capacity of corneal endothelial cells[J]. Biomedicine & Pharmacotherapy, 2021, 141: 111834.
Wang X, Zhou Q, Zhao C, et al. Multiple roles of FGF10 in the regulation of corneal endothelial wound healing[J]. Experimental Eye Research, 2021, 205: 108517.
Hsueh Y J, Meir Y J J, Lai J Y, et al. Ascorbic acid ameliorates corneal endothelial dysfunction and enhances cell proliferation via the noncanonical GLUT1-ERK axis[J]. Biomedicine & Pharmacotherapy, 2021, 144: 112306.
Park J H, Kim M, Chuck R S, et al. Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells[J]. Scientific reports, 2021, 11(1): 6250.
Shan K, Qiu J, Zhou R, et al. RNA-seq identifies long non-coding RNAs as potential therapeutic targets for human corneal endothelial dysfunction under oxidative stress[J]. Experimental Eye Research, 2021, 213: 108820.
Kim D J, Jung M Y, Pak H J, et al. Development of a novel hyaluronic acid membrane for the treatment of ocular surface diseases[J]. Scientific reports, 2021, 11(1): 2351.
Kim D J, Jung M Y, Park J H, et al. Moxifloxacin releasing intraocular implant based on a cross-linked hyaluronic acid membrane[J]. Scientific reports, 2021, 11(1): 24115.
Hsueh Y J, Meir Y J J, Lai J Y, et al. Lysophosphatidic acid improves corneal endothelial density in tissue culture by stimulating stromal secretion of interleukin‐1β[J]. Journal of Cellular and Molecular Medicine, 2020, 24(12): 6596-6608.
Yu F, Zhang W, Yan C, et al. PAX6, modified by SUMOylation, plays a protective role in corneal endothelial injury[J]. Cell Death & Disease, 2020, 11(8): 683.
Hsueh Y J, Meir Y J J, Yeh L K, et al. Topical ascorbic acid ameliorates oxidative stress-induced corneal endothelial damage via suppression of apoptosis and autophagic flux blockage[J]. Cells, 2020, 9(4): 943.
Zhang W, Yu F, Yan C, et al. PTEN inhibition accelerates corneal endothelial wound healing through increased endothelial cell division and migration[J]. Investigative Ophthalmology & Visual Science, 2020, 61(8): 19-19.
Hsueh Y J, Ma D H K, Ma K S C, et al. Extracellular matrix protein coating of processed fish scales improves human corneal endothelial cell adhesion and proliferation[J]. Translational vision science & technology, 2019, 8(3): 27-27.
Li D, Zhang P. Protective effect and molecular mechanism of liquiritin on oxybuprocaine-induced apoptosis of human corneal endothelial cells[J]. Experimental and therapeutic medicine, 2018, 15(4): 3432-3438.
Wang Q, Wei C, Ma L, et al. Inflammatory cytokine TNF-α promotes corneal endothelium apoptosis via upregulating TIPE2 transcription during corneal graft rejection[J]. Graefe's Archive for Clinical and Experimental Ophthalmology, 2018, 256: 709-715.
Chen X, Wu L, Li Z, et al. Directed differentiation of human corneal endothelial cells from human embryonic stem cells by using cell-conditioned culture media[J]. Investigative ophthalmology & visual science, 2018, 59(7): 3028-3036.
Kim J Y, Park J H, Kim M, et al. Safety of nonporous silica nanoparticles in human corneal endothelial cells[J]. Scientific reports, 2017, 7(1): 14566.
Yao Q, Zhang W, Hu Y, et al. Electrospun collagen/poly (L-lactic acid‑co‑ε‑caprolactone) scaffolds for conjunctival tissue engineering[J]. Experimental and therapeutic medicine, 2017, 14(5): 4141-4147.
Chen J, Yan C, Zhu M, et al. Electrospun nanofibrous SF/P (LLA-CL) membrane: a potential substratum for endothelial keratoplasty[J]. International journal of nanomedicine, 2015: 3337-3350.
Hsueh Y J, Chen H C, Wu S E, et al. Lysophosphatidic acid induces YAP-promoted proliferation of human corneal endothelial cells via PI3K and ROCK pathways[J]. Molecular Therapy-Methods & Clinical Development, 2015, 2.

For research use only. Not for any other purpose.

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How are quality control metrics and benchmarks established and monitored in cell biology?

Metrics such as cell viability, confluency, passage number, and contamination rates are routinely monitored to establish benchmarks for quality control in cell biology experiments.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Promising results

We were able to use this tumor cell product to test the efficacy of various cancer treatments, with promising results.

29 Mar 2023

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