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HCEC-B4G12

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  • Specification
  • Technical Resources
  • Recommended Products
  • Cat.No.
  • CSC-C3457
  • Description
  • HCEC-B4G12 is a clonal subpopulation started in 2005 from the parental cell line HCEC-12 established from normal cells of the posterior epithelium of the cornea of a 91-year-old Caucasian woman transformed with the early region of the SV40 genome including genes encoding large T-antigen and small t-antigen; the subclone is described to represent differentiated cells of the so-called corneal endothelium
  • Recommended Medium
  • Human-Endothelial-SFM + 10 ng/ml FGF-2 (observe the shelf-life of the medium; do not apply h.i. FBS in order to keep differentiation status)
  • Organism
  • Human
  • Morphology
  • cuboid epitheloid cells growing strongly adherent in monolayers
  • Culture Properties
  • monolayer
  • Source
  • corneal endothelium
  • Quality Control
  • Mycoplasma: negative in microbiological culture, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 (+), cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
    Viruses: P
  • Storage and Shipping
  • Frozen with 90% medium (including FGF-2), 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com