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KYSE-150
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Cat.No.
CSC-C0423
Description
Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice
Species
Human
Source
esophageal squamous cell carcinoma
Recommended Medium
Culture Properties
monolayer
Morphology
epitheloid cells growing as adherent monolayer
Karyotype
Human hypertriploid karyotype with 12% polyploidy - 70(68-75)<3n>XderX, -X, +1, -3, -4, -8, -9, +11, -14, -15, -16, -18, -21, +12mar (4xbisat), add(X)(q27), inv(1)(p34q32), del(1)(p32), del(1)(q11), del(7)(q32), del(8)(p21), i(9q), add(9)(q34), add(11)(p1
Quality Control
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cy
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cy
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
Datasheet- Q & A
- Customer Review
Q:
What should I be aware of before and after thawing the serum?
A:
When melting, it is better to put it at 4℃ first. After melting, the serum should not be stored at 4°C for a long time and should be used as soon as possible.
Average Rating: 4.0 | 1 Scientist has reviewed this product
High-quality
The cell products were of high quality and made our experiments simpler to repeat and troubleshoot.
19 June 2021
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