KYSE-150

Cat.No.: CSC-C0423

Species: Human

Source: esophageal squamous cell carcinoma

Morphology: epitheloid cells growing as adherent monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0423

Description

Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice

Species

Human

Source

esophageal squamous cell carcinoma

Recommended Medium

49%RPMI1640 + 49%Ham's F-12 + 2%h.i. FBS.

Culture Properties

monolayer

Morphology

epitheloid cells growing as adherent monolayer

Karyotype

Human hypertriploid karyotype with 12% polyploidy - 70(68-75)<3n>XderX, -X, +1, -3, -4, -8, -9, +11, -14, -15, -16, -18, -21, +12mar (4xbisat), add(X)(q27), inv(1)(p34q32), del(1)(p32), del(1)(q11), del(7)(q32), del(8)(p21), i(9q), add(9)(q34), add(11)(p1

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cy

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What should I be aware of before and after thawing the serum?

When melting, it is better to put it at 4℃ first. After melting, the serum should not be stored at 4°C for a long time and should be used as soon as possible.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

High-quality

The cell products were of high quality and made our experiments simpler to repeat and troubleshoot.

19 June 2021

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