Peripheral Blood Mononuclear Cells
Peripheral blood mononuclear cells (PBMCs) are cells with a single, round nucleus and are collected from the peripheral or circulating blood by density centrifugation with Ficoll, a polysaccharide. PBMCs include dendritic cells, monocytes, and lymphocytes (T, B, and NK cells). T cells are CD3+ and further classified into helper T cells (TH) that express CD4 and cytotoxic T cells (TC) that express CD8. B cells are characterized by CD19 expression and respond to pathogens by producing a large number of antigen-specific antibodies to neutralize foreign objects. NK cells are CD56+CD3-, which originate from the same progenitor cells as T and B cells, but lack the antigen receptor. Finally, monocytes are the largest type of PBMCs and express CD14. They circulate in the bloodstream for 1-3 days and then usually move into tissues throughout the body, where they can differentiate into macrophages and myeloid-lineage dendritic cells.
| Cell Type | Percentage in PBMCs | Markers | Function |
| CD4+ T cells | 25-60% | CD3+CD4+ | Coordinate adaptive immunity through activation and regulation of other immune cells |
| CD8+ T cells | 5-30% | CD3+CD8+ | Destroy cancer cells or cells that are infected or damaged |
| B cells | 5-10% | CD19+ | Secrete antibodies as part of humoral immune response |
| NK cells | 10-30% | CD56+CD3- | Trigger lysis or apoptosis of infected cells |
| Monocytes | 5-10% | CD14+ | Take up foreign objects via phagocytosis, perform antigen presentation, and produce cytokines |
| Dendritic cells | 1-2% | Process and present antigen material to T cells |
PBMCs are a powerful tool for research and clinical studies related to human health and disease. By efficient and successful processing and analysis of PBMCs, researchers and clinicians can evaluate immune responses, gain a deeper understanding of the immune system, and apply their findings to the treatment of human diseases. PBMC are now readily available from many commercial sources, with samples isolated from both healthy and diseased individuals.
Filters Clear all filters
Species
- Bovine (19)
- Cat (1)
- Chicken (3)
- Dog (110)
- Fish (1)
- Fruitfly (1)
- Goat (2)
- Guinea Pig (5)
- Hamster (95)
- Horse (1)
- Human (770)
- Monkey (129)
- Mouse (855)
- Pig (106)
- Rabbit (248)
- Rat (318)
- Sheep (2)
- Squirrel (1)
- Turkey (1)
Source
- Adipose (27)
- Adrenal Gland (10)
- Airway (6)
- Anus (3)
- Aorta (82)
- Artery (178)
- Bile Duct (9)
- Bladder (45)
- Blood (184)
- Bone (13)
- Bone Marrow (154)
- Brain (155)
- Breast (60)
- Bronchus (42)
- Cartilage (18)
- Cervix (5)
- Chorion (5)
- Choroid (8)
- Ciliary Body (1)
- Colon (60)
- Conjunctiva (8)
- Cord Blood (24)
- Cornea (24)
- Dental Pulp (4)
- Dermis (108)
- Diaphragm (3)
- Ear (9)
- Embryo (21)
- Endometrium (8)
- Epidermis (24)
- Epididymis (3)
- Esophagus (31)
- Eye (87)
- Foreskin (2)
- Gallbladder (4)
- Gingiva (18)
- Hair Follicle (15)
- Heart (59)
- Intestine (147)
- Iris (1)
- Kidney (143)
- Lens (4)
- Liver (117)
- Lung (180)
- Lymph Node (26)
- Mesentery (18)
- Nose (2)
- Oral Cavity (8)
- Ovary (68)
- Oviduct (6)
- Pancreas (64)
- Pancreatic Duct (3)
- Pancreatic Islet (11)
- Parathyroid Gland (4)
- Penis (6)
- Perineurium (1)
- Periodontal Ligament (5)
- Periodontium (23)
- Peripheral Blood (145)
- Peritoneal Cavity (14)
- Placenta (29)
- Prostate (60)
- Rectum (3)
- Retina (36)
- Salivary Gland (3)
- Sclera (3)
- Seminal Vesicle (1)
- Skeletal Muscle (35)
- Skin (150)
- Small Intestine (54)
- Spinal Cord (10)
- Spleen (71)
- Stomach (36)
- Synovial Fluid (2)
- Synovium (9)
- Tendon (6)
- Testis (14)
- Thymus (47)
- Thyroid (34)
- Tongue (4)
- Tonsil (3)
- Tooth (4)
- Trabecular Meshwork (2)
- Trachea (40)
- Umbilical Cord (25)
- Ureter (8)
- Uterus (56)
- Vas Deferens (1)
- Vein (101)
Cell Type
- Adipocyte (3)
- Astrocyte (32)
- B Cell (30)
- Basal Cell (3)
- Basophil (1)
- Beta Cell (3)
- Cardiomyocyte (11)
- CD133+ Cell (6)
- CD34+ Cell (21)
- Cholangiocyte (9)
- Chondrocyte (13)
- Dendritic Cell (15)
- Endothelial Cell (675)
- Endothelial Progenitor Cell (7)
- Eosinophil (1)
- Epithelial Cell (475)
- Fibroblast (424)
- Fibroblasts (2)
- Glial Cell (55)
- Granule Cell (2)
- Granulocyte (12)
- Hepatic Stellate Cell (9)
- Hepatocyte (23)
- Interstitial Cell (9)
- Keratinocyte (22)
- Keratocyte (3)
- Kupffer Cell (8)
- Leydig Cell (3)
- Lymphocyte (82)
- Macrophage (30)
- Mast Cell (3)
- Melanocyte (11)
- Meningeal Cell (4)
- Mesangial Cell (10)
- Mesothelial Cell (4)
- Microglia (6)
- Microvascular Cell (306)
- Monocyte (15)
- Mononuclear Cell (105)
- Myeloid Cell (2)
- Myoblast (5)
- Myofibroblast (3)
- Myosatellite Cell (2)
- Neuron (45)
- Neutrophil (9)
- NK Cell (11)
- Oligodendrocyte (3)
- Oligodendrocyte Progenitor Cell (3)
- Osteoblast (8)
- Osteoclast (2)
- Osteocyte (3)
- Pancreatic Stellate Cell (4)
- Pericyte (20)
- Podocyte (4)
- Preadipocyte (16)
- Progenitor Cell (14)
- Red Blood Cell (11)
- Retinal Ganglion Cell (3)
- Satellite Cell (2)
- Schwann Cell (4)
- Sebocyte (1)
- Sertoli Cell (5)
- Skeletal Muscle Cell (9)
- Smooth Muscle Cell (233)
- Spermatogonium (3)
- Stromal Cell (39)
- Synoviocyte (7)
- T Cell (37)
- Tenocyte (6)
- Trabecular Meshwork Cell (2)
- Trophoblast (4)
Disease
- Acute Lymphocytic Leukemia (ALL) (15)
- Acute Myeloid Leukemia (AML) (13)
- Amyotrophic Lateral Sclerosis (ALS) (4)
- Aplastic Anemia (AA) (1)
- Arteriovenous Malformation (AVM) (1)
- Asthma (5)
- Astrocytoma (2)
- Autoimmune Hemolytic Anemia (AIHA) (1)
- Autoimmune Lymphoproliferative Syndrome (ALPS) (1)
- Breast Cancer (5)
- Cancer (124)
- Cervical Cancer (2)
- Chronic Lymphocytic Leukemia (CLL) (19)
- Chronic Myeloid Leukemia (CML) (14)
- Chronic Obstructive Pulmonary Disease (COPD) (6)
- Colon Cancer (6)
- Crohn's Disease (3)
- Cystic Fibrosis (CF) (5)
- Diabetes (110)
- Diabetes Type 1 (16)
- Diabetes Type 2 (18)
- Diffuse Large B-Cell Lymphoma (4)
- Dilated Cardiomyopathy (DCM) (1)
- Duchenne Muscular Dystrophy (DMD) (5)
- Essential Thrombocythemia (ET) (1)
- Glioblastoma (3)
- Guillain-Barre Syndrome (GBS) (1)
- Hypertension (27)
- Idiopathic Thrombocytopenic Purpura (ITP) (1)
- Inflammatory Bowel Disease (IBD) (5)
- Iron-Deficiency Anemia (1)
- Kidney Cancer (2)
- Legg–Calvé–Perthes Disease (LCPD) (2)
- Leukopenia (1)
- Liver Cancer (2)
- Lung Cancer (7)
- Mantle Cell Lymphoma (MCL) (8)
- Melanoma (2)
- Mucopolysaccharidosis (2)
- Multiple Myeloma (MM) (12)
- Multiple Sclerosis (MS) (3)
- Muscular Dystrophy (MD) (1)
- Myelodysplastic Syndrome (MDS) (3)
- Neurofibromatosis (NF) (3)
- Non-Hodgkin Lymphoma (NHL) (10)
- Normal (2316)
- Osteoarthritis (OA) (4)
- Ovarian Cancer (4)
- Pancreatic Cancer (1)
- Pancytopenia (1)
- Parkinson's Disease (PD) (2)
- Plasmacytoma (1)
- Polycythemia (1)
- Prostate Cancer (4)
- Psoriasis (4)
- Rheumatoid Arthritis (RA) (6)
- Robertsonian Translocation (ROB) (1)
- Sickle Cell Anemia (2)
- Systemic Lupus Erythematosus (SLE) (4)
- Thrombocytopenia (1)
- Transverse Myelitis (TM) (1)
- Ulcerative Colitis (UC) (2)
- Waldenström Macroglobulinemia (WM) (2)
Description: PB-Mononuclear Cells are isolated from Acute Lymphoid Leukemia peripheral blood by diluting the ...
Description: PB-Mononuclear Cells are isolated from Acute Lymphoid Leukemia peripheral blood by diluting the ...
Description: Human CD3+ pan T cells from Creative Bioarray are selected from peripheral blood mononuclear cells ...
Description: Human peripheral blood regulatory T cells from Creative Bioarray are a subpopulation of T cells ...
Description: Human peripheral blood CD4+CD45RA+ naïve helper T cells Creative Bioarray are enriched by depleting ...
Description: Human CD4+CD45RO+ memory T cells from Creative Bioarray are isolated from peripheral blood ...
Description: Human peripheral blood CD4+ helper T cells from Creative Bioarray are enriched by means of negative ...
Description: Human peripheral blood CD8+CD45RA+ naïve cytotoxic T cells from Creative Bioarray are enriched by ...
Description: Human CD8+ cytotoxic T cells from Creative Bioarray are isolated from peripheral blood mononuclear ...
Description: Human CD14+ monocytes from Creative Bioarray are isolated from peripheral blood mononuclear cells ...
Description: Human CD19+CD27+ memory B cells from Creative Bioarray are isolated from peripheral blood ...
Description: Human CD19+CD27– naïve B cells from Creative Bioarray are isolated from peripheral blood ...
Description: Human CD36+ erythroid progenitor cells from Creative Bioarray are derived from cultured peripheral ...
Description: Origin: HumanDesease: Rheumatoid Arthritis (RA)Application: These cells can be used to investigate ...
Description: Origin: HumanDesease: Multiple Sclerosis (MS)Application: These cells can be used to investigate ...
Description: Origin: HumanDesease: Osteoarthritis (OA)Application: These cells can be used to investigate the ...
Description: Origin: HumanDesease: Acute Lymphoblastic Leukemia (ALL)Application: These cells can be used to ...
Description: Origin: HumanDesease: Acute Myeloid Leukemia (AML)Application: These cells can be used to ...