Peripheral Blood Mononuclear Cells
Peripheral blood mononuclear cells (PBMCs) are cells with a single, round nucleus and are collected from the peripheral or circulating blood by density centrifugation with Ficoll, a polysaccharide. PBMCs include dendritic cells, monocytes, and lymphocytes (T, B, and NK cells). T cells are CD3+ and further classified into helper T cells (TH) that express CD4 and cytotoxic T cells (TC) that express CD8. B cells are characterized by CD19 expression and respond to pathogens by producing a large number of antigen-specific antibodies to neutralize foreign objects. NK cells are CD56+CD3-, which originate from the same progenitor cells as T and B cells, but lack the antigen receptor. Finally, monocytes are the largest type of PBMCs and express CD14. They circulate in the bloodstream for 1-3 days and then usually move into tissues throughout the body, where they can differentiate into macrophages and myeloid-lineage dendritic cells.
| Cell Type | Percentage in PBMCs | Markers | Function |
| CD4+ T cells | 25-60% | CD3+CD4+ | Coordinate adaptive immunity through activation and regulation of other immune cells |
| CD8+ T cells | 5-30% | CD3+CD8+ | Destroy cancer cells or cells that are infected or damaged |
| B cells | 5-10% | CD19+ | Secrete antibodies as part of humoral immune response |
| NK cells | 10-30% | CD56+CD3- | Trigger lysis or apoptosis of infected cells |
| Monocytes | 5-10% | CD14+ | Take up foreign objects via phagocytosis, perform antigen presentation, and produce cytokines |
| Dendritic cells | 1-2% | Process and present antigen material to T cells |
PBMCs are a powerful tool for research and clinical studies related to human health and disease. By efficient and successful processing and analysis of PBMCs, researchers and clinicians can evaluate immune responses, gain a deeper understanding of the immune system, and apply their findings to the treatment of human diseases. PBMC are now readily available from many commercial sources, with samples isolated from both healthy and diseased individuals.
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Species
- Bovine (19)
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Disease
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Description: MPB-CD133+ Stem/Progenitor cells are positively isolated from MPB-Mononuclear Cells by using a ...
Description: Mantle Cell Lymphoma-Plasma is obtained by centrifugation of blood tissues. Mantle Cell ...
Description: Mantle Cell Lymphoma-Plasma is obtained by centrifugation of blood tissues. Mantle Cell ...
Description: Mantle Cell Lymphoma-PB-Mononuclear Cells are density separated by Ficoll-Paque from Mantle Cell ...
Description: Mantle Cell Lymphoma-PB-Mononuclear Cells are density separated by Ficoll-Paque from Mantle Cell ...
Description: Mantle Cell Lymphoma-Bone Marrow-Mononuclear Cells are density separated by Ficoll-Paque to enrich ...
Description: Mantle Cell Lymphoma-Bone Marrow-Mononuclear Cells are density separated by Ficoll-Paque to enrich ...
Description: NPB-Macrophages are derived from negatively-selected peripheral blood monocytes and stimulated with ...
Description: These mononuclear cells are sero-typed for HLA-A2. Mononuclear cells (Mononuclear Cells) are ...
Description: Untouched NPB-CD56+ Natural Killer (NK) cells are negatively isolated from mononuclear cells using ...
Description: Untouched NPB-CD14+ Monocytes are indirectly isolated from mononuclear cells using an ...
Description: Positively Selected NPB-CD56+ NK cells are directly isolated from mononuclear cells using direct ...
Description: Positively-Selected NPB-CD14+ Monocytes are directly isolated from mononuclear cells using an ...
Description: Diffuse Large B-Cell Lymphoma-PB-Plasma is obtained by centrifugation of peripheral blood tissue. ...
Description: Diffuse Large B-Cell Lymphoma-PB-Mononuclear Cells are density separated using Ficoll-Paque. ...
Description: Crohn's Disease peripheral blood plasma is obtained by centrifugation of the PB. This product has a ...
Description: Peripheral blood mononuclear cells are isolated from Crohn's Disease peripheral blood by diluting ...
Description: Cord Blood-Plasma is obtained by centrifugation of whole cord blood tissue. This product has a ...