Human Umbilical Mesenchymal Stem Cells (HUMSC)

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Cat.No.

CSC-7700W

Description

Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. This property, in combination with MSC's developmental plasticity, has generated tremendous interest in the potential use of MSC to replace damaged tissues. MSC isolated from Wharton's jelly of umbilical cords were induced to transform into neurons and glia in vitro through stepwise culturing in neuron-conditioned medium, sonic hedgehog, and FGF-8 ; to cardiomyocytes by treating then with 5-azacytidine or by culturing them in cardiomyocytes-conditioned medium; and to adipogenic and osteogenic lineage. They express matrix receptors CD44 and CD10^5, but not hematopoietic lineage marker CD34, and a significant quantity of mesenchymal stem cell markers SH2 and SH3.HUMSC from Bioarray Research Laboratories are isolated from Wharton's jelly of umbilical cords. HUMSC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HUMSC are characterized by immunofluorescent method with antibodies to CD73, CD90 and CD105. HUMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUMSC are guaranteed to further culture at the conditions provided by Bioarray Research Laboratories.

Species

Human

Recommended Medium

SuperCult® Complete Mesenchymal Stem Cell Medium

Storage and Shipping

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Human Umbilical Mesenchymal Stem Cells (HUMSCs) are typically derived from Wharton's jelly in the discarded post‑delivery umbilical cord, representing a source of multipotent stromal cells that is both abundant and ethically non‑controversial. Following enzymatic or explant digestion, the isolated cells adhere rapidly to plastic, attain a fibroblast‑like, spindle‑shaped morphology and form a homogenous monolayer in 2-4 days. Phenotypically, HUMSCs express the classical MSC markers CD73, CD90, CD105 and CD44 while being negative for hematopoietic antigens such as CD34, CD45 and HLA‑DR, consistent with a mesenchymal phenotype. In culture, HUMSCs can differentiate into osteoblasts, chondrocytes and adipocytes, as well as neural‑like, cardiomyocyte‑like and hepatocyte‑like cells, showing the potential for broad multilineage differentiation. In addition, they also display robust immunomodulatory properties in vitro. For example, they suppress the proliferation of PHA‑stimulated T cells, and down‑regulate the production of IFN‑γ while up‑regulating the production of anti‑inflammatory mediators such as IL‑10, TGF‑β1, PGE₂ and IDO.

Pre‑clinical studies have found HUMSCs promote the repair of bone and cartilage, and aid in myocardial regeneration and neural tissue repair. HUMSCs have also been investigated in clinical trials for use in the treatment of graft‑versus‑host disease, atopic dermatitis, arthritis and other conditions. The combination of low immunogenicity, robust expansion and potent paracrine activity has made HUMSCs a promising cellular platform for regenerative medicine and immune‑modulating treatments.

Umbilical cord-derived mesenchymal stem cells (UC-MSCs) were cultured in Cytomix medium (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and their adipogenic, osteogenic, and chondrogenic differentiation was studied using Oil Red O, Alkaline Phosphatase, and Alcian Blue, respectively. — Fig. 1. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) were cultured in Cytomix medium (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and their adipogenic, osteogenic, and chondrogenic differentiation was studied using Oil Red O, Alkaline Phosphatase, and Alcian Blue, respectively (Kacham S, Bhure TS, *et al.*, 2021).

Xenograft of Human Umbilical Mesenchymal Stem Cells Promotes Recovery from Chronic Ischemic Stroke in Rats

Stroke is one of the leading causes of adult disability. In cases of chronic stroke, no therapy is available, with only rehabilitation as an intervention option. In an earlier study, intravenous injection of human umbilical mesenchymal stem cells (HUMSCs) in the acute phase after ischemic stroke promoted functional recovery in rats. Fu's team aimed to evaluate HUMSCs xenograft as a potential treatment for chronic stroke in rats.

The rotarod test showed that in the Normal + Saline and Normal + HUMSCs groups, retention time changed little and was not statistically significant. In the Stroke + Saline and Stroke + HUMSCs groups, retention time dropped to 21-23% of normal on day 1 post-stroke, indicating impaired mobility due to cerebral ischemia. This persisted until day 14. By day 21, the Stroke + Saline group's retention time was 27% of normal, with no improvement by day 56. However, the Stroke + HUMSCs group's retention time increased to 49% on day 21, significantly higher than the Stroke + Saline group, and this trend continued until day 56. Although the Stroke + HUMSCs group's retention time increased from day 21 to day 42, it remained lower than the Normal groups (p < 0.05) (Fig. 1C). In the cylinder test, all groups had about 50% contralateral forelimb use before surgery. In the Normal + Saline and Normal + HUMSCs groups, forelimb use remained unchanged until day 56 (Fig. 1D).

HUMSCs transplantation enhanced motor function in rats with chronic stroke. — Fig. 1. HUMSCs transplantation enhanced motor function in rats with chronic stroke (Fu Y-S, Yeh C-C, *et al.*, 2022).

Transplantation of HUMSCs, but Not ADMSCs, Improved Arterial Oxygen Saturation (SpO₂) in Rats with PF

Pulmonary fibrosis (PF) is a progressive illness with various causes and limited effective treatments. Chu's team compared the effectiveness of human mesenchymal stem cells from umbilical cord Wharton's jelly (HUMSCs) and adipose tissue (ADMSCs) in reversing pulmonary fibrosis in rats.

SpO₂ was measured via pulse oximetry. In the Normal group, mean SpO₂ was 97.0-99.0%. The Injury group's SpO₂ dropped to 81.6 ± 0.5% on Day 7 and stayed around 82.2 ± 0.8% until Day 49, significantly lower than the Normal group (Fig. 2A, B). The Injury+ADMSCs and Injury+HUMSCs groups had similar SpO₂ levels to the Injury group from Day 7 to Day 21. The Injury+ADMSCs group showed no increase in SpO₂ from Day 28 to Day 49. The Injury+HUMSCs group's SpO₂ increased significantly to 85.3 ± 0.7% on Day 28 and reached 92.3 ± 0.7% by Day 49, better than the Injury and Injury+ADMSCs groups. Despite this increase, the Injury+HUMSCs group's SpO₂ remained lower than the Normal group (Fig. 2A, B).

Transplantation of HUMSCs, but not ADMSCs, improved pulmonary function in rats with PF. — Fig. 2. Transplantation of HUMSCs, but not ADMSCs, improved pulmonary function in rats with PF (Chu K-A, Yeh C-C, *et al.*, 2023).

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