QuantiGene Plex Assay
QuantiGene Plex Assay is a hybridization-based assay, which utilizes the xMAP LuminexTM magnetic beads and branched DNA signal amplification technology. The assay is based on direct quantification of the miRNA/mRNA/DNA targets using xMAP Luminex beads for multiplexing and the probe hybridize to target sequeces within the sample, elimating the need for RNA isolation and reverse transcription. Apart from that, this assay can be used to qualify gene transcripts from different types of sample, including blood, fresh tissue, FFPE tissue, purifed RNA or cultured cells.
QuantiGene Plex Assay provides high-throughout quantitative gene expression analysis to support a wide range of applications, including multiple focus areas in oncology; gastrointetinal stromal tumors, lung, prostrate, breast, colorectal and pancreatic cancers. Creative Bioarray provides customizable QuantiGene Plex Assay for you to rapidly chracterize miRNA, mRNA or DNA targets for you.
Figure 1. A. Branched DNA signal amplification on Luminex beads; B. Quantification of fold change in prostate cancer markers.Cancer and normal tissues from the same FFPE blocks were macro-dissected and dissolved in homogenizing solution using the QuantiGene 2.0 Assay.
Features and advantages
- 3 to 80 RNA targets
- Direct RNA Quantification
- RNA purification, cDNA synthesis, and PCR amplification not required
- 2,400 x signal ampilification per copy
QuantiGene Plex Assay available for many sample types, shown below:
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QuantiGene Plex Assay available for many species, shown below:
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Quotation and ordering
Our customer service representatives are available 24hr a day! We thank you for choosing Creative Bioarray at your preferred QuantiGene Plex Assay.
References
- Preuss S B. Expression of the Arabidopsis thaliana BBX32 gene in soybean increases grain yield[J]. PloS one, 2012, 7(2): e30717.
- Ting D T. Aberrant overexpression of satellite repeats in pancreatic and other epithelial cancers[J]. Science, 2011, 331(6017): 593-596.
- Furusato B. ERG oncoprotein expression in prostate cancer: clonal progression of ERG-positive tumor cells and potential for ERG-based stratification[J]. Prostate cancer and prostatic diseases, 2010, 13(3): 228.
- Hall J S. Exon-array profiling unlocks clinically and biologically relevant gene signatures from formalin-fixed paraffin-embedded tumour samples[J]. British journal of cancer, 2011, 104(6): 971.
