Induced Pluripotent Stem Cells (iPSCs) are a type pf pluripotent stem cell artificially derived, and often referred to as programmed, from adult somatic cells using the expression of certain genes in culture. Using adult somatic cells, they have been reprogrammed to an embryonic stem cell-like state by being forced to express genes and factors important for maintaining undifferentiated embryonic stem cells. Since the discovery that human iPSCs could be generated by inducing expression of the four reprogramming factors (OCT-4, SOX-2, KLF-4 and c-MYC) many different reprogramming technologies have emerged to generate iPSCs, each possessing their own advantages and disadvantages.
IPSCs removed the ethical concerns associated with embryonic stem cells and allowed scientists to model human diseases that were previously impossible to model such as Alzheimer’s, Parkinson’s and Autism. Creative Bioarray offers a broad range of kits and related reagents that are useful to the stem cell scientist interested to perform reprogramming trials.
QualiStem® Episomal iPSC Reprogramming Kit
Creative Bioarray QualiStem® Episomal iPSC Reprogramming Kit is produced from standardized protocols using highly efficient set of essential reprogramming factors’ episomal vectors that is sufficient for generating high numbers of human iPSCs using the transfection step.
With QualiStem® Episomal iPSC Reprogramming Kit, you can:
- Rapid transgene-free, virus-free, and footprint-free iPSCs generation
- Safe for all stages of your research from basic research to pre-clinical research
- Used with multiple cell types
- High efficient reprogramming
- Allows the reprogramming of a variety of somatic cell types and provides flexibility in somatic cell selection
QualiStem® RNA iPSC Reprogramming Kit
Creative Bioarray QualiStem® RNA iPSC Reprogramming Kit is produced from standardized protocols using a single stand RNA vector that contains necessary reprogramming factors.
With QualiStem® RNA iPSC Reprogramming Kit, you can:
- Rapid transgene-free, virus-free, and footprint-free iPSCs generation
- No screening required to ensure viral remnants are not present
- High efficient and rapid reprogramming
- A single transfection step (Only 1-day transfection required)
- iPSCs generation in both feeder and feeder-free conditions
QualiStem® Retrovirus iPSC Reprogramming Kit
QualiStem® Retrovirus iPSC Reprogramming Kit is produced from standardized protocols using a highly efficient retrovirus cocktail including Oct4 retrovirus, Sox2 retrovirus, Klf4 retrovirus, c-Myc retrovirus, which is sufficient for generating high numbers of human iPSCs using the transfection step. Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. The QualiStem® Retrovirus iPSC Reprogramming Kit offers such opportunity to generate iPSCs from various tissues and cell types.
QualiStem® Lentivirus iPSC Reprogramming Kit
Creative Bioarray QualiStem® Lentivirus iPSC Reprogramming Kit is produced from standardized protocols using a highly efficient set of lentivirus containing Oct4, Sox2, Klf4, c-Myc genes, which is sufficient for generating high numbers of human iPSCs using the transfection step.
QualiStem® iPSC Protein Reprogramming Kit
Protein-based human iPSCs system is the ultimate choice for iPSCs generation that is not require the destruction of ex utero embryos and completely eliminates possible genome mutations and insertions associated with DNA transfections or viral transduction. One of key challenges in protein-based iPSC generation is the low efficiency of protein transduction of target cells. Creative Bioarray offers optimized protein transfection reagents that can enhance protein transduction efficiency by up to 70%.
