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Pluripotent stem cells (PSCs) have the unique capability to proliferate indefinitely and to differentiate into all cell types. iPSCs were found to be very similar to ESCs, which ESC-like colony morphology and expression of pluripotency markers. During the reprogramming process, the transcriptional network changes gradually from the somatic to the pluripotent state. The fully reprogrammed cells showing the most ES cell like patterns of gene expression, and partially reprogrammed cells showing intermediate phenotypes. The classical markers for the pluripotent embryonic state, such as alkaline phosphatase activity or TRA expression, are critical for tracking cell reprogramming and for gaining confidence in the dedifferentiated status of the reprogrammed cells.
Creative Bioarray offers many iPS cell characterization tools for your research.
Alkaline Phosphatase Staining Assay
Since the alkaline phosphatase is a stem cell membrane marker, the elevated expression of this protein is a demonstration of undifferentiated, pluripotent state. Ready-to-use Alkaline Phosphatase Staining Kit for the identification of alkaline phosphatase activity.
Pluripotency Markers (Protein)
There are several biomarkers can be used to determine pluripotency of iPS cells. Ready-to-use iPSC Immunocytochemistry Kit and Human iPSC Flow cytometry Kit for the identification of pluripotent iPSCs by immunocytochemical and flow cytometry analysis.
Pluripotency Markers (mRNA)
Ready-to-use Human iPSC qPCR Kit and Mouse iPSC qPCR Kit to confirm the iPSC with well-established pluripotency markers.