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Peripheral Blood Mononuclear Cells

Peripheral blood mononuclear cells (PBMCs) are cells with a single, round nucleus and are collected from the peripheral or circulating blood by density centrifugation with Ficoll, a polysaccharide. PBMCs include dendritic cells, monocytes, and lymphocytes (T, B, and NK cells). T cells are CD3+ and further classified into helper T cells (TH) that express CD4 and cytotoxic T cells (TC) that express CD8. B cells are characterized by CD19 expression and respond to pathogens by producing a large number of antigen-specific antibodies to neutralize foreign objects. NK cells are CD56+CD3-, which originate from the same progenitor cells as T and B cells, but lack the antigen receptor. Finally, monocytes are the largest type of PBMCs and express CD14. They circulate in the bloodstream for 1-3 days and then usually move into tissues throughout the body, where they can differentiate into macrophages and myeloid-lineage dendritic cells.

Cell TypePercentage in PBMCsMarkersFunction
CD4+ T cells25-60%CD3+CD4+Coordinate adaptive immunity through activation and regulation of other immune cells
CD8+ T cells5-30%CD3+CD8+Destroy cancer cells or cells that are infected or damaged
B cells5-10%CD19+Secrete antibodies as part of humoral immune response
NK cells10-30%CD56+CD3-Trigger lysis or apoptosis of infected cells
Monocytes5-10%CD14+Take up foreign objects via phagocytosis, perform antigen presentation, and produce cytokines
Dendritic cells1-2%
Process and present antigen material to T cells

PBMCs are a powerful tool for research and clinical studies related to human health and disease. By efficient and successful processing and analysis of PBMCs, researchers and clinicians can evaluate immune responses, gain a deeper understanding of the immune system, and apply their findings to the treatment of human diseases. PBMC are now readily available from many commercial sources, with samples isolated from both healthy and diseased individuals.

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Description: MPB-CD133+ Stem/Progenitor cells are positively isolated from MPB-Mononuclear Cells...

Cat#: CSC-C4473X INQUIRY

Description: Mantle Cell Lymphoma-Plasma is obtained by centrifugation of blood tissues. Mantle...

Cat#: CSC-C4475X INQUIRY

Description: Mantle Cell Lymphoma-Plasma is obtained by centrifugation of blood tissues. Mantle...

Cat#: CSC-C4476X INQUIRY

Description: Mantle Cell Lymphoma-PB-Mononuclear Cells are density separated by Ficoll-Paque from...

Cat#: CSC-C4477X INQUIRY

Description: Mantle Cell Lymphoma-PB-Mononuclear Cells are density separated by Ficoll-Paque from...

Cat#: CSC-C4478X INQUIRY

Description: Mantle Cell Lymphoma-Bone Marrow-Mononuclear Cells are density separated by Ficoll-Paque...

Cat#: CSC-C4481X INQUIRY

Description: Mantle Cell Lymphoma-Bone Marrow-Mononuclear Cells are density separated by Ficoll-Paque...

Cat#: CSC-C4482X INQUIRY

Description: NPB-Macrophages are derived from negatively-selected peripheral blood monocytes and...

Cat#: CSC-C4483X INQUIRY

Description: These mononuclear cells are sero-typed for HLA-A2. Mononuclear cells (Mononuclear...

Cat#: CSC-C4485X INQUIRY

Description: Untouched NPB-CD56+ Natural Killer (NK) cells are negatively isolated from mononuclear...

Cat#: CSC-C4486X INQUIRY

Description: Untouched NPB-CD14+ Monocytes are indirectly isolated from mononuclear cells using...

Cat#: CSC-C4490X INQUIRY

Description: Positively Selected NPB-CD56+ NK cells are directly isolated from mononuclear cells...

Cat#: CSC-C4491X INQUIRY

Description: Positively-Selected NPB-CD14+ Monocytes are directly isolated from mononuclear cells...

Cat#: CSC-C4493X INQUIRY

Description: Diffuse Large B-Cell Lymphoma-PB-Plasma is obtained by centrifugation of peripheral...

Cat#: CSC-C4504X INQUIRY

Description: Diffuse Large B-Cell Lymphoma-PB-Mononuclear Cells are density separated using Ficoll-Paque...

Cat#: CSC-C4505X INQUIRY

Description: Crohn's Disease peripheral blood plasma is obtained by centrifugation of the PB....

Cat#: CSC-C4512X INQUIRY

Description: Peripheral blood mononuclear cells are isolated from Crohn's Disease peripheral blood...

Cat#: CSC-C4513X INQUIRY

Description: Cord Blood-Plasma is obtained by centrifugation of whole cord blood tissue. This...

Cat#: CSC-C4515X INQUIRY

For research use only. Not for any other purpose.