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Peripheral Blood Mononuclear Cells

Peripheral blood mononuclear cells (PBMCs) are cells with a single, round nucleus and are collected from the peripheral or circulating blood by density centrifugation with Ficoll, a polysaccharide. PBMCs include dendritic cells, monocytes, and lymphocytes (T, B, and NK cells). T cells are CD3+ and further classified into helper T cells (TH) that express CD4 and cytotoxic T cells (TC) that express CD8. B cells are characterized by CD19 expression and respond to pathogens by producing a large number of antigen-specific antibodies to neutralize foreign objects. NK cells are CD56+CD3-, which originate from the same progenitor cells as T and B cells, but lack the antigen receptor. Finally, monocytes are the largest type of PBMCs and express CD14. They circulate in the bloodstream for 1-3 days and then usually move into tissues throughout the body, where they can differentiate into macrophages and myeloid-lineage dendritic cells.

Cell TypePercentage in PBMCsMarkersFunction
CD4+ T cells25-60%CD3+CD4+Coordinate adaptive immunity through activation and regulation of other immune cells
CD8+ T cells5-30%CD3+CD8+Destroy cancer cells or cells that are infected or damaged
B cells5-10%CD19+Secrete antibodies as part of humoral immune response
NK cells10-30%CD56+CD3-Trigger lysis or apoptosis of infected cells
Monocytes5-10%CD14+Take up foreign objects via phagocytosis, perform antigen presentation, and produce cytokines
Dendritic cells1-2%
Process and present antigen material to T cells

PBMCs are a powerful tool for research and clinical studies related to human health and disease. By efficient and successful processing and analysis of PBMCs, researchers and clinicians can evaluate immune responses, gain a deeper understanding of the immune system, and apply their findings to the treatment of human diseases. PBMC are now readily available from many commercial sources, with samples isolated from both healthy and diseased individuals.

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Description: Peripheral blood mononuclear cells are isolated from Ulcerative Colitis peripheral...

Cat#: CSC-C4397X INQUIRY

Description: Type II Diabetes peripheral blood plasma is obtained by centrifugation of the Type...

Cat#: CSC-C4398X INQUIRY

Description: Peripheral blood mononuclear cells are isolated from Type II Diabetes peripheral...

Cat#: CSC-C4399X INQUIRY

Description: Type I Diabetes peripheral blood plasma is obtained by centrifugation of the Type...

Cat#: CSC-C4400X INQUIRY

Description: Peripheral blood mononuclear cells are isolated from Type I Diabetes peripheral blood...

Cat#: CSC-C4401X INQUIRY

Description: Systemic Lupus Erythematosus (SLE) peripheral blood plasma is obtained by centrifugation...

Cat#: CSC-C4402X INQUIRY

Description: Peripheral blood mononuclear cells are isolated from Systemic Lupus Erythematosus...

Cat#: CSC-C4403X INQUIRY

Description: Sickle Cell Anemia (SCA) peripheral blood plasma is obtained by centrifugation of...

Cat#: CSC-C4404X INQUIRY

Description: Peripheral blood mononuclear cells are isolated from Sickle Cell Anemia (SCA) peripheral...

Cat#: CSC-C4405X INQUIRY

Description: Rheumatoid Arthritis (RA) peripheral blood plasma is obtained by centrifugation of...

Cat#: CSC-C4415X INQUIRY

Description: Peripheral blood mononuclear cells are isolated from Rheumatoid Arthritis (RA) peripheral...

Cat#: CSC-C4416X INQUIRY

Description: PV-PB-Mononuclear Cells are enriched by separation using Ficoll-Paque density gradient...

Cat#: CSC-C4417X INQUIRY

Description: PV-PB-Mononuclear Cells are enriched by separation using Ficoll-Paque density gradient...

Cat#: CSC-C4418X INQUIRY

Description: Psoriasis peripheral blood plasma is obtained by centrifugation of the Psoriasis...

Cat#: CSC-C4419X INQUIRY

Description: Peripheral blood mononuclear cells are isolated from Psoriasis peripheral blood by...

Cat#: CSC-C4420X INQUIRY

Description: Osteoarthritis peripheral blood plasma is obtained by centrifugation of the Osteoarthritis...

Cat#: CSC-C4422X INQUIRY

Description: Peripheral blood mononuclear cells are isolated from Osteoarthritis peripheral blood...

Cat#: CSC-C4423X INQUIRY

Description: NPB Plasma is obtained from healthy donors through the COBE Spectra apheresis machine...

Cat#: CSC-C4424X INQUIRY

For research use only. Not for any other purpose.