SuperQuick® In Vitro Mammalian Cell Gene Mutation Test Kit (HGPRT)

GTK-YS008

Under normal physiological conditions, cells express hypoxanthine-guanine phosphoribosyltransferase (HGPRT), which catalyzes the conversion of 6-thioguanine (6-TG) into toxic nucleotides such as nucleoside-5'-monophosphates (NMPs). These metabolites are incorporated into DNA, leading to cell death. However, upon exposure to carcinogens and/or mutagens, mutations may occur in the structural gene for HGPRT located on the X chromosome, resulting in a loss of HGPRT activity. These mutant cells become resistant to 6-TG and are able to survive and proliferate in selective medium containing 6-TG.
In this assay, cultured mammalian cells are exposed to the test substance—with or without an exogenous metabolic activation system—for a defined period. Following treatment, cells are subcultured and then plated in selective medium containing 6-TG. Mutant cells capable of 6-TG resistance will form discrete colonies, which are counted to determine the mutation frequency, thereby evaluating the mutagenic potential of the test article.

In vitro Genotoxicity Studies on food, drugs, chemicals, cosmetics, health products, pesticides, medical devices, etc.

Chinese hamster lung cell line (V79)
S9 Mix
S9 Reaction Buffer
THMG (100×)
THG (100×)
6-TG
3-MCA
MMC

20 mL*36 tests

Store at -70°C.

Dry ice delivered.

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