Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra and interchain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse and rat tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Rat PlGF shares 60% amino acid identity with human PlGF2. The gene for PlGF has been mapped to mouse chromosome 12, human chromosome 14 and rat chromosome 6. PlGF binds with highaffinity to Flt1, but not to Flk1/KDR. The full ORF of rat PLGF (Met1-Leu158) was cloned from total RNA of rat sinusoidal endothelial cells using standard protocols. The native protein expressed in insect cells starts with Ala24. The recombinant rat PLGF-2 is a 15.14 kDa protein containing 135 amino acid residues.
Rat Pgf expressed in Insect cells
CAT# CSC-CTK0863-5 (5 μg); CAT# CSC-CTK0863-25 (25 μg)
Greater than 95% as determined by SDS-PAGE analysis.
Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant rat PLGF can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.1 - 5 ng/mL.
Less than 1 EU/μg.
Lyophilized from a steril-filtered protein solution in 25 mM Tris (pH 8.5).
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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