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Species: human (Homo sapiens)
Cell type: natural killer lymphoma
Origin: established from the peripheral blood of a 50-year-old man with non-Hodgkin lymphoma (large granular lymphocytic) in 1992; cells were described as having azurophilic granula and strong cytotoxic NK activity
peripheral blood
Recommended Medium
75% SuperCult® AlphaMEM without Nucleosides without L-Glutamine + 12.5% h.i. FBS + 12.5% horse serum + 2 mM L-glutamine + 0.2 mM inositol + 0.1 mM 2-mercaptoethanol + 0.02 mM folic acid + 100-200 U/ml recombinant IL-2
Culture Properties
cells growing in clumps in suspension
STR DNA Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,12
D16S539: 11,12
D5S818: 12,13
D7S820: 10,11
THO1: 6,9.3
vWA: 18
Human hypotetraploid karyotype with 4% polyploidy
CD2 +, CD7 +, CD11a +, CD28 + , CD45 +, CD54 +, CD56 +, CD1 -, CD3 -, CD4 -, CD5 -, CD8 -, CD10 -, CD14 -, CD16 -, CD19 -, CD20 -, CD23 -, CD34 -, HLA-DR -
a. Natural killer related reserach
b. Tumor immunotherapy related research
c. Cell-Based assay
d. In vivo efficacy study
Quality Control
Mycoplasma: Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Selective biochemical test: negative
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Storage and Shipping
Ship in dry ice.
Store in liquid nitrogen.
BioSafety Level
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.,
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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