Interleukin 12 (IL-12) is a cytokine with important regulatory functions bridging innate and adaptive immunity. It has been proposed as an immune adjuvant for vaccination therapy of infectious diseases and malignancies. The inflammatory properties of IL-12 play an important role in the adjuvant. Interleukin 12 (IL-12) is a proinflammatory cytokine composed by two chains, p40 and p35. It plays a key role in the promotion of a Th1 immune response in the gastrointestinal mucosa. An enhanced expression of IL-12 mRNA in gastric mucosa has been reported in individuals infected by Helicobacter pylori. Recombinant rat IL-12, produced in Sf9- insect cells, is a glycosylated disulfide linked heterodimeric polypeptide of 503 amino acids and a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
Rat Il12 expressed in Sf9-insect cells
CAT# CSC-CTK0824-10 (10 μg); CAT# CSC-CTK0824-50 (50 μg)
Greater than 95% as determined by SDS-PAGE analysis.
Recombinant rat IL-12 is fully biologically active when compared to a standard. The ED50 range of 0.01-0.05 ng/ml was determined by the dose-dependent secretion of IFN-gamma from PHA and IL-2-activated murine splenocyes that have been co-stimulated with PMA. Optimal concentration should be determined for each individual application by a dose-response assay.
Less than 1 EU/μg.
Sterile-filtered white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) solution in water with no additives.
Please centrifuge product before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-12 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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