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ActoFactor™ Recombinant Rat Interferon gamma

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Cat.No.
CSC-CTK0811
Description
Recombinant Rat Interferongamma (rRa IFN-gamma) is a lymphoid factor, which influences antigen presenting cells and stimulates a number of lymphoid cell functions including the anti- microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Creative Bioarray's Rat IFN-gamma is a 15.6 kDa protein containing 135 amino acid residues.
Species
Rat
Product Overview
Rat Ifng expressed in E.coli
Molecular Mass
15.6 kDa
Size
CAT# CSC-CTK0811-100 (100 μg); CAT# CSC-CTK0811-500 (500 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and HPLC analysis.
Activity
The ED50 as determined by a cytopathic effect inhibition assay with murine L929 cells challenged with EMC virus was found to be ≤ 0.1 ng/ml, corresponding to a specific activity of Greater than 1 x 10^7 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered, concentrated protein solution in PBS (pH 7.4).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted IFN-gamma should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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