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ActoFactor™ Recombinant Mouse Prolactin

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Cat.No.
CSC-CTK0656
Description
Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.|Recombinant mouse Prolactin produced in E. coli is a single, non-glycosylated polypeptide chain of 198 amino acids having a molecular mass of 22.5 kDa. Prolactin has been purified by using proprietary chromatographic techniques.
Species
Mouse
Product Overview
Mouse Prl expressed in E.coli
Molecular Mass
22.5 kDa
Size
CAT# CSC-CTK0656-50 (50 μg); CAT# CSC-CTK0656-250 (250 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma Nb2-11 was found to be < 0.065 ng/ml.
Endotoxin Level
N/A
Formulation
Lyophilized from a concentrated (1 mg/ml), sterile- filtered protein solution with in 10 mM sodium phosphate buffer (pH 8) and 50 mM Nacl with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at –20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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