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ActoFactor™ Recombinant Mouse Nerve growth factor

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Cat.No.
CSC-CTK0757
Description
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this genes expression is associated with allergic rhinitis. Nerve Growth Factor was the first protein found from the family of neurotrophic factors that influence the growth and differentiation of sympathetic and sensory neurons. NGF consists of 3 different subunits: alpha, beta, and gamma. The beta subunit is accountable for its growth stimulating activity. The synthesis of NGF in astrocytes is enhanced by a range of cytokines such as IL-1, TNF- , PDGF & TGF- . Recombinant mouse beta-NGF produced in E. coli is a non-glycosylated, noncovalently disulfide-linked homodimer containing 2 identical chains of 120 amino acids each and having a molecular mass of 13,471 Dalton each.
Species
Mouse
Product Overview
Mouse Ngf expressed in E.coli
Molecular Mass
N/A
Size
CAT# CSC-CTK0757-20 (20 μg); CAT# CSC-CTK0757-100 (100 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
b-NGF is fully biologically active when compared to standards. Activity was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line (TF-1). The ED50 for this effect is 0.2 ng/ml corresponding to a specific activity of 5 x 10^6 IU/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a protein concentration without additives.
Reconstitution
Please centrifuge product briefly before opening vial. This solution can be diluted into other aqueous buffers and stored at -20 C° for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu beta-NGF beta should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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