Recombinant mouse Interleukin-4 (rMu IL-4) is a potent lymphoid cell growth factor, which stimulates the growth and survivability of certain B cells and T cells. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant mouse IL-4 is a 13.5 kDa globular protein containing 120 amino acid residues.
Mouse Il4 expressed in E.coli
CAT# CSC-CTK0683-20 (20 μg); CAT# CSC-CTK0683-100 (100 μg)
Greater than 98% as determined by SDS-PAGE, FPLC and HPLC analysis.
Recombinant IL-4 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent proliferation of Murine HT-2 cells is ≤ 2 ng/ml, corresponding to a specific activity of Greater than 5 x 10^5 units/mg.
Less than 1 EU/μg.
Sterile-filtered through a 0.2 micron filter and lyophilized with no additives.
Please centrifuge product briefly before opening the vial. The lyophilized recombinant mouse IL-4 should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted mouse IL-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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