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ActoFactor™ Recombinant Mouse Growth hormone
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Cat.No.
CSC-CTK0781
Description
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. Mouse recombinant Growth Hormone produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids and having a molecular mass of 22 kDa.
Species
Mouse
Product Overview
Mouse Gh expressed in E.coli
Molecular Mass
22 kDa
Size
CAT# CSC-CTK0781-100 (100 μg); CAT# CSC-CTK0781-500 (500 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
Recombinant mouse Growth Hormone is fully biologically active when compared to a standard Human Growth Hormone (3 units/mg).
Endotoxin Level
N/A
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a protein solution in 50 mM Tris-HCl, 150 mM NaCl buffer (pH 8).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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