FGF-15 takes part in the suppression of bile acid biosynthesis by down-regulation of CYP7A1 expression. FGF-15 is expressed in the developing brain. FGF-15 produced in E. coli is a 23.78 kDa protein containing 203 amino acid residues including a 10 a.a. N-Terminal His-tag.
Mouse Fgf15 expressed in E.coli
CAT# CSC-CTK0667-10 (10 μg); CAT# CSC-CTK0667-50 (50 μg)
Greater than 90% as determined by SDS-PAGE analysis.
Less than 1 EU/μg.
Lyophilized from a filtered (0.4 @m) protein soluti on (0.5 mg/ml) in 20 mM TRIS (pH 8.0) and 20 mM NaCl.
Please centrifuge product before opening vial. It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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