Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimotos thyroiditis, Gravesdisease and Type 1 diabetes mellitus. The chemokine IP-10 (interferon-inducible protein of 10 kDa, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells. Recombinant mouse IP-10 produced in E. coli is a single, non-glycosylated polypeptide chain of 77 amino acids and a molecular mass of 8,701 Dalton.
Mouse Cxcl10 expressed in E.coli
CAT# CSC-CTK0739-25 (25 μg); CAT# CSC-CTK0739-125 (125 μg)
Greater than 97% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Recombinant murine IP-10 is fully biologically active when compared to a standard. The biological activity is calculated by its ability to chemoattract IL-2 activated T cells at concentrations ranging between 0.1-10 ng/ml.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized with no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu IP-10 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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