MIP-1b is a member of the b (CC) family of chemokines. It is produced by fibroblasts, monocytes, T and B lymphocytes, neutrophils, smooth muscle cells, mast cells and tumor cell lines. MIP-1b is a chemoattractant for monocytes and T cells, preferentially CD4+. In addition, MIP-1b potentiates early hematopoietic precursors growth. MIP-1b binds to CCR1, CCR5 and US28 receptors. The recombinant protein encoding the mature polypeptide contains 69 amino acid residue and migrates with an apparent Mr of 7.8 kDa.
Mouse Ccl4 expressed in E.coli
A DNA sequence encoding the mature mouse MIP-1b protein was expressed in E. coli.
CAT# CSC-CTK0042-10 (10 μg); CAT# CSC-CTK0042-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by ability to chemoattract mouse BaF/3 cells transfected with hCCR5 and the ED50 for this effect was typically 3 - 12 ng/mL.
Endotoxin level less than 0.1 ng per 1 μg of the cytokine as determined by the LAL method.
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile, 0.1% TFA containing 50 μg of bovine serum albumin per 1 μg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted mouse MIP-1b/CCL4 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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