Prolactin is a pituitary hormone that plays a role in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The primary step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. Prolactin is a hormone involved in a range of significant functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. Prolactin exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane Prolactin receptor. PRLR varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL-R consists of at least 3 separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function. The recombinant human extracellular domain Prolactin Receptor produced in E. coli is a non- glycosylated, polypeptide chain containsing of 210 amino acids and having a molecular mass of 23.97 kDa. It is purified by proprietary chromatographic techniques according to Bignon et al. (1994) JBC 269; 3318-24, and tested according to Gertler et al. (1996) JBC 271; 24482-91.
Human PRLR expressed in E.coli
CAT# CSC-CTK0102-20 (20 μg); CAT# CSC-CTK0102-100 (100 μg)
Greater than 97% as determined by SDS-PAGE, SEC-HPLC analysis and non-denaturing gel
The biological activity is determined by the dose-dependant inhibition of Prolactin-stimulated proliferation of Nb2 cells and by high affinity binding of ovine Prolactin and other lactogenic hormones in 1:1 molar ratio.
Sterile-filtered, white, lyophilized (freeze-dried)powder. The protein was lyophilized from a concentrated (0.4 mg/ml) solution with 0.0045 mM NaHCO .
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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