IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3 region and to the upstream INS gene at the 5 region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Recombinant human Pro-IGF-2 produced in HEK cells is a glycosylated monomer, having a total molecular weight of 25 kDa. It has been purified by proprietary chromatographic techniques.
Human IGF2 expressed in HEK cells
CAT# CSC-CTK0209-10 (10 μg); CAT# CSC-CTK0209-50 (50 μg)
Greater than 95% as determined by SDS-PAGE analysis.
rHu Pro-IGF-2 is fully biologically active when compared to a standard. The specific activity was determined by the dose-dependent stimulation of the proliferation of MCF-7 cells (human breast cancer cell line) and is typically 2-8 ng/ml.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.
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