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ActoFactor™ Recombinant Human Melanoma inhibitory activity

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Cat.No.
CSC-CTK0456
Description
MIA acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion. In a study of human melanoma cell lines with different metastatic capacity MIA mRNA expression appeared to be inversely correlated with pigmentation. MIA has been shown to represent a very sensitive and specific serum marker for systemic malignant melanoma that might be useful for staging of primary melanomas, detection of progression from localized to metastatic disease during follow- up, and monitoring therapy of advanced melanomas. Recombinant human MIA produced in E. coli is a single, non-glycosylated polypeptide chain of of 108 amino and a total molecular mass of 12,237 Dalton. The Melanoma Inhibitory protein (MIA) was identified as an inhibitor of in vitro growth of malignant melanoma cells. The protein contains a SH3 domain.
Species
Human
Product Overview
Human MIA expressed in E.coli
Molecular Mass
12.24 kDa
Size
CAT# CSC-CTK0456-5 (5 μg); CAT# CSC-CTK0456-25 (25 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Activity
The biological activity is calculated by the inhibiting effect on the invasion of Mel In Tumor cells (Bosserhoff et al.2003 Lab Invest. 83, 1583-94) and found active in Mel In assay.
Endotoxin Level
Less than 1 EU/μg.
Formulation
The protein was lyophilized from a concentrated (1.66 mg/ml) solution containing 20 mM potassium phosphate (pH 7.0) and 150 mM potassium chloride.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu MIA should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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